不同低氧训练模式对大鼠肝脏及肾脏组织内自由基代谢的影响  

Effect of Different Hypoxic Training Methods on Free Radical Metabolism of Liver and Kidney Tissue in Mice

作　　者：李洁[1] 张耀斌[1] 邢良美[1] 

机构地区：[1]西北师范大学体育学院,甘肃兰州730070

出　　处：《体育科学》2008年第5期56-61,68,共7页China Sport Science

摘　　要：目的:观察不同低氧训练模式对大鼠肝脏及肾脏组织中MDA含量、SOD活性、GSH-px活性及CAT活性的影响,探讨不同低氧训练模式下机体自由基代谢的变化规律,为确定低氧训练方案提供实验依据。方法:40只大鼠随机分为5组(n=8),常氧训练组(living low-training low,LL)、高住高练组(living high-training high,HH)、高住低训组(living high- training low,HL)、低住高练组(living low-training high,LH)、高住高练低训组(living high-ex- ercise high-training low,HHL)。分光光度法测定组织匀浆液中MDA含量、SOD活性、GSH- px活性和CAT活性。结果:1)与LL组相比:各低氧训练组肝脏组织中SOD活性、GSH-px活性及CAT活性均显著性增高(P<0.001或P<0.05);MDA含量也均显著性增高(P<0.001)。2)与LL组相比:各低氧训练组肾脏组织中MDA含量均显著性增高(P<0.001);HH组和HHL组SOD活性、GSH-px活性及CAT活性均显著性增高(P<0.001或P<0.05);HL组SOD活性及CAT活性均显著性增高(P<0.001或P<0.05),GSH-px活性无显著性差异;LH组SOD活性及GSH-px活性均显著性增高(P<0.001或P<0.05),CAT活性显著性降低(P<0.001)。结论:高住高练、高住低训、低住高练和高住高练低训模式,均可显著性增强力竭运动后即刻肝脏和肾脏组织中的抗氧化能力,但仍无法彻底清除运动中产生的自由基,导致自由基脂质过氧化损伤较常氧训练加重。低氧与运动对肝脏和肾脏组织自由基脂质过氧化损伤有协同作用。The purpose of this study was to observe the effects of different hypoxic training methods on the activity of superoxide dismutase （SOD）, glutathione peroxidase （GSH-px）, catalase （CAT） and amount of malondialdehyde （MDA） of liver and kidney tissue in mice and try to find the changeable regularity of free radical metabolism in order to provide practical basis for hypoxic training. 40 healthy two-month-old male Wister rats were randomly divided in- to 5 groups as living low-training low （LL）, living high-training high （HH）, living high- training low （HL）, living low-training high （LH）, and living high-exercise high-training low （HHL）. Spectrophotometric analysis was applied to evaluate activity of SOD, GSH-px, CAT and amount of MDA in homogenized. The result showed that 1. Compared with LL, rats from HH, HL, LH and HHL showed a significant increase of SOD, GSH-px and CAT activities and MDA amount in liver tissue（P〈0. 001 or P〈0.05）. 2. Compared with LL, rats from HH, HL,LH and HHL showed a significant increase of MDA amount, HH and HHL showed a significant increase of SOD, GSH-px and CAT activities, HL presented a significant increase of SOD and CAT activities and no distinct change of GSH-px activities, LH expressed a significant increase of SOD and GSH-px activities and a significant decrease of CAT activities in kid- ney tissue（P〈0. 001 or P〈0.05）. It concluded that HH, HL, LH and HHL could improve antioxidant power in liver and kidney tissues immediately after exhausting exercise, but it did not completely eliminate free radical. However,impairment by lipid peroxidation was more se- rious than that by LL. There was better function of cooperation between hypoxic and training to free radical lipid peroxidative damages in liver and kidney tissues.

关 键 词：低氧训练 肝脏 肾脏 自由基 鼠 动物实验 

分 类 号：G804.7[文化科学—运动人体科学]