毛细管电泳-安培检测法同时测定中成药珍菊降压片中有效成分  

Simultaneous Determination of Active Ingredients in "Zhen Ju" Hypertension Pills by Capillary Electrophoresis with Amperometric Detection

作　　者：丁飞[1] 陈黎明[2] 

机构地区：[1]上海市环境科学研究院,上海200233 [2]上海市计量测试技术研究院,上海200233

出　　处：《理化检验（化学分册）》2008年第5期430-432,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：用毛细管电泳-安培检测法实现了中成药珍菊降压片中有效成分盐酸可乐定、氢氯噻嗪和芦丁的同时检测。在25 mmol.L-1Na2B4O7-50 mmol.L-1NaH2PO4缓冲溶液中,3种电活性物质在15 min内实现完全分离。每片珍菊降压片中3种有效成分盐酸可乐定、氢氯噻嗪和芦丁的含量差别很大分别为0.03,5,20 mg。通过改变样品稀释溶剂,调整了3种活性物质在电极上的电流响应,实现了珍菊降压片中含量差别百倍以上的3种电活性物质的同时检测,测定结果与该药片的每片标示值之间的相对偏差均小于2%。Capillary electrophoresis and amperometric detection （CE-AD） was applied to the separation and simultaneous determination of clonidine hydrochloride, hydrochlorothiazide and rutin, 3 active ingredients of the ready-prepared Chinese medicine ＂Zhen Ju＂ hypertension pills. In a buffer medium of 25 mmol· L^-1 Na2B4O7 and 50 mmol·L^-1 NaH2PO4 with its original pH value of 7. 9, three electroactive substances mentioned above were separated completely within 15 rain. The large differences in concentrations of the 3 compounds （0. 03, 5 and 20 mg per pill respectively） in the hypertension pills made it difficult to the simultaneous determination. The difficulty was overcome by choosing water as a diluent for the sample solution, thus making a reasonable adjustment of the current responses of the 3 ingredients at the carbon electrode, and the simultaneous determination was made possible, giving values of relative deviation less than 2%, from the labelled values of each pill.

关 键 词：毛细管电泳 安培检测 盐酸可乐定 芦丁 氢氯噻嗪 

分 类 号：O657.3[理学—分析化学]