急性长时间运动对大鼠心肌线粒体NO、NOS水平及线粒体功能的影响  

Effects of an Acute Bout of Strenuous Exercise on the Level of NO/NOS in Rat's Myocardium Mitochondria and Mitochondrial

作　　者：高彩暇[1] 丁树哲[2] 许其卫[1] 

机构地区：[1]淮阴师范学院体育系,江苏淮安223001 [2]华东师范大学体育与健康学院,上海200062

出　　处：《中国体育科技》2008年第3期105-110,共6页China Sport Science and Technology

摘　　要：12只8周龄雌性SD大鼠随机分成对照组(6只)和运动组(6只),运动组负重游泳299±29min后立即处理,取心肌线粒体测定线粒体RCR,随后测定心肌总NOS、mtNOS活性,心肌三型NOSmRNA表达水平,心肌细胞和线粒体中NO的含量,线粒体抑制活性氧的能力以及[Ca^(2+)]i。结果发现,一次性长时间运动后,尽管大鼠心肌线粒体抗氧化能力下降,但线粒体呼吸功能完好,线粒体游离钙离子浓度没有发生变化,这可能和心肌及心肌线粒体NO含量、NOS活性水平下降有关。Twelve 8-week-old female SD rats are randomly divided into control group （six） and exercise group （six） and let the rats of exercise group swim with heavy in 299±29 min averagely, then killed to get myocardium mitochondria to measure mitochondria RCR, total NOS, mtNOS activity of myocardium, the express level of myocardium Ⅲ NOSmRNA, NO amount in myocardium cells and mitochondria, the ability of mitochondria＇ s restrain to radical oxygen species and [Ca^2＋ ]i. The result shows that, although the ability of rats＇ mitochondrial restrain to reactive oxygen species （O2^- ,·OH） decreases, rats ＇ myocardium mitochondrial respiration controlling rate is complete, the concentration of mitochondria free Ca^2＋ is not changed. This is related to descend of contend of myocardium cells and rnitochondria NO, and related to descend of mtNOS activity.

关 键 词：运动 线粒体呼吸控制率 NO NOS 活性氧 游离钙离子 鼠 动物实验 

分 类 号：G804.7[文化科学—运动人体科学]