川贝母中非生物碱类成分的研究  

Non-alkaloid Constituents in Fritillaria cirrhosa and Fritillaria delarayi

作　　者：曹新伟[1,2] 陈四保[1,2] 陈士林[1,2] 肖培根[1] 李军[3] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100094 [2]深圳市中药药学及分子药理学研究重点实验室,深圳518057 [3]香港科技大学生物系暨中药研发中心,中国香港九龙

出　　处：《世界科学技术-中医药现代化》2008年第2期83-88,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金项目(30572324)濒危品种川贝母光合蒸腾特性与野生抚育生态因子相关性研究;国家自然科学基金项目(30530860)中国重要药用植物类群亲缘学研究

摘　　要：目的:对川贝母Fritillaria cirrhosa D.Don和梭砂贝母Friltillaria delavayi Franch.的干燥鳞茎进行化学成分研究。方法:利用硅胶柱色谱及Sephadex LH-20柱色谱进行分离纯化,根据理化性质和波谱数据鉴定化合物的结构。结果:从川贝母中共分离鉴定了:β-谷甾醇(β-sitosterol,),单棕榈酸甘油酯(2-monopalmitin,)等12个化合物。从梭砂贝母中共分离鉴定了E-对-羟基肉桂酸甲酯(E-p-hydrox- ycinnamic acid methyl ester,16)等13个化合物。结论:有15个化合物为首次从贝母属中分离得到,β-谷甾醇和胡萝卜苷为首次从川贝母中分离得到,3个化合物为首次从梭砂贝母中分离得到。We studied the chemical constituents of the bulbs of Fritillaria cirrhosa and Fritillaria delavayi. In the study, chemical constituents were isolated using column chromatographic methods, including silica gel and sephadex LH-20, and their structures were elucidated based on the physicochemical and spectral evidences. Twelve compounds were isolated from F. cirrhosa, and their structures were identified as 15-sitosterol （1）, E-cinnamic acid （2）, 2-monopalmitin （3）, daucosterol （4）, uracil （5）, thymine （6）, cytidine （7）, inosine （8）, uridine （9）, guanosine （10）, thymidine （11）, adenosine （12）. Thirteen compounds were isolated from F. delavay,i and their structures were identified as E-3,4,5- trimethoxycinnamic acid （13）, E-p-methoxycinnamic acid （14）, E-cinnamic acid （2）, E-p-hydroxycinnamic acid （15）, E-p-hydroxycinnamic acid methyl ester （16）, ferulic acid （17）, caffeic acid （18）, 1-O-feruloylglycerol （19）, β-sitosterol （1）, daucosterol （4）, uracil （5）, thymine （6）, adenosine （12）. Compounds 2,3,5 - 10 and 13 - 19 were obtained for the first time from the genus, while compounds 1 and 4 were obtained from F. cirrhosa. Compounds 1, 4 and 12 were obtained for the first time from F. delavayi.

关 键 词：贝母属 川贝母 梭砂贝母 化学成分 

分 类 号：R284[医药卫生—中药学] R282.710.3[医药卫生—中医学]