汉防己甲素联合屈洛昔芬对K562和K562／A02细胞NF-κB蛋白表达的影响  

Effect of tetrandrine in combination with droloxifen on the expression of NF-κB protein in K562 and K562/A02 cell lines

作　　者：苏爱玲[1] 陈宝安[1] 黄成垠[1] 高峰[1] 程坚[1] 许文林[2] 沈惠玲[2] 孙新臣[1] 成红艳[1] 丁家华[1] 高冲[1] 孙耘玉[1] 王骏[1] 赵刚[1] 陈宁娜[1] 赵慧慧[1] 

机构地区：[1]东南大学附属中大医院血液科,南京210009 [2]镇江市第一人民医院

出　　处：《中华血液学杂志》2008年第5期321-324,共4页Chinese Journal of Hematology

基　　金：国家自然科学基金（39970832）

摘　　要：目的研究汉防己甲素（Tet）和屈洛昔芬（DRL）单独及联合用药后对K562细胞及其耐药细胞株K562／A02的核因子κB（NF-κB）表达的影响，以进一步探讨其逆转多药耐药的作用机制。方法采用免疫细胞化学及Western blotting法分别检测Tet（1μmol／L）、DRL（5μmol／L）单独及联合作用于K562和K562／A02细胞后NF-κB核转位水平和胞核NF-κB蛋白表达水平的变化。结果①K562／A02细胞NF-κB核转位水平[（65．0±4．2）％]及胞核NF-κB蛋白表达（3．545±0．219）明显高于K562细胞[（39．6±0．9）％和2．366±0．141]（P〈0．01）；②Tet、DRL单独及联合作用6、12h，对K562细胞NF-κB核转位水平及胞核NF-κB蛋白表达水平无明显影响（P〉0．05）；③两药单独及联合作用6、12h，可明显降低K562／A02细胞NF-κB蛋白核转位水平和胞核NF-κB蛋白表达水平（P〈0．05和P〈0．01），两药联合作用增强（P〈0．05），作用12h较作用6h效果更显著（P〈0．05）。结论K562／A02细胞的胞核NF-κB蛋白表达水平明显高于K562细胞，NF-κB的活化可能参与了K562／A02细胞耐药的发生；抑制NF—κB活化可能参与了Tet和DRL逆转K562／A02细胞多药耐药过程。Objective To study the effect of tetrandrine （Tet） in combination with droloxifen （DRL） on the expression of nuclear factor kappa B（NF-κB） in K562 and K562/A02 cell lines and its reversal mechanism. Methods The activation of NF-κB in K562 and K562/A02 cell lines and the effect of Tet or DRL alone or in combination on NF-κB protein expression were determined with immunocytochemistry and Western blotting respectively. Results 1.K562/A02 cells displayed higher level of NF-κB protein expression than K562 cells. 2.The application of Tet or DRL alone or in combination had no effect on NF-κB expression in K562 cells at 6 h and 12 h （ P 〉 0.05 ）. 3.Tet and DRL alone or in combination could significantly down-regulate NF-κB protein expression in nuclei of K562/A02 cells. The effect was more significant in combination than either alone. This effect was more significant at 12 h than at 6 h. Conclusions 1.Activation of NF-κB may be involved in the mechanism of MDR of K562/A02 cell line. 2.Inhibition of NF-κB activation may be involved in the reversal of multidrug resistance in K562/A02 cells by Tet and DRL.

关 键 词：抗药性 多药 汉防己甲素 屈洛昔芬 核因子NF-ΚB 

分 类 号：R686[医药卫生—骨科学]