JWA基因-76G>C和454C>A多态性与宫颈癌易感性  

JWA gene -76G>C and 454C>A polymorphisms significantly increase the risk of cervical cancer in Jiangsu population

作　　者：朱慧[1] 王美林[2] 李春平[2] 李爱萍[2] 沈洪兵[2] 韩素萍[1] 

机构地区：[1]南京医科大学第一附属医院妇产科,江苏南京210029 [2]南京医科大学公共卫生学院分子毒理学实验室,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2008年第4期462-466,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：南京医科大学科技创新基金(CX2004006)

摘　　要：目的:探讨JWA基因多态性与江苏人群宫颈癌易感性之间的关系。方法:采用聚合酶链反应限制性片段长度多态性(PCR-RFLP)方法,检测227例宫颈癌患者和333例年龄、性别相匹配的非肿瘤者JWA-76G>C、454C>A和723T>G多态性的频率和分布,比较不同基因型携带者患宫颈癌的危险性,通过分层分析探讨初潮年龄、初次生育年龄和产次对患宫颈癌的影响。结果:JWA-76GC和454AA基因型均可增加罹患宫颈癌的危险性。携带3～5个危险等位基因者比携带0～2个等位基因者患宫颈癌的危险性更大(OR=1.70,95%CI=1.20～2.42)。分层分析结果显示,初潮年龄早、生育年龄晚和产次多且携带3～5危险等位基因者能增加患宫颈癌的危险性。本研究未发现JWA基因723T>G多态性与宫颈癌之间存在显著相关。结论:JWA基因-76G>C和454C>A多态性显著增加江苏地区汉族人群患宫颈癌的危险性。Objective ：To investigate the association between of JWA gene single nucleotide polymorphisms and the risk of cervical cancer. Methods：In a hospital-based case-control study of 227 cervical patients and 333 cancer-free controls frequency-matched by age,the genotype of the -76G〉C,454C〉A and 723T〉G polymorphisms of JWA gene were detected by the polymerase chain reaction- restriction fragment length polymorphism （PCR-RFLP） method;the interaction of menarche age,reproductive age for the first time （RAFT） and parity were further analyzed. Results： A significant increased risk of cervical cancer was found to be associated with the -76GC genotype compared with the -76GG genotype （OR=1.84,95% CI=1.21-2.78）,and 454AA genotype compared with the 454CC genotype（OR=1.84,95% CI=1.08-3.14）. Moreover,individuals with 3-5 risk alleles will increase the risk of cervical cancer accompanied with early menarche age,late RAFT and many parity. There was no association found between JWA 723T〉G polymorphism and cervical cancer. Conclusion： The JWA-76G〉C and 454C〉A polymorphisms might positively contribute to the etiology of cervical cancer.

关 键 词：JWA基因 宫颈癌 基因多态性 分子流行病学 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]