肝移植术后HBV再感染患者HBVX区基因变异研究  

Mutation of X region nucleotide sequence of hepatitis B virus DNA in patients with HBV reinfection after liver transplantation

作　　者：沈中阳[1] 宋红丽[1] 郑虹[1] 王建[1] 郑卫萍[1] 王政禄[1] 张帆[1] 

机构地区：[1]天津市第一中心医院器官移植中心,300192

出　　处：《中华普通外科杂志》2008年第5期368-371,共4页Chinese Journal of General Surgery

基　　金：天津市科委基础重点课题资助项目（05YFJZC01302）;天津市卫生局科技基金课题资助项目（局02KY02）

摘　　要：目的分析肝移植术后HBV再感染患者血清乙型肝炎病毒（hepatitis B viral，HBV）DNAX区基因的变异情况。方法选择2002年6月至2003年12月期间因乙肝相关终末期肝病在本院行肝移植手术的320例患者，移植术后患者血清采用聚合酶链反应（PCR）法扩增HBV DNA X区，并做直接测序分析。术后随访1．5—3年。结果本组11例患者肝移植前、后HBV X区基因序列都有程度不等的点突变（5—39），有6例术前与术后同时具有nt1762（A→T）、nt1764（G→A）变异，11例患者同时存在nt1636-nt1741多处位点变异。结论HBV DNA X区核苷酸nt1762、nt1764双位点变异在肝移植术后HBV再感染患者中是常见现象，免疫抑制剂不能改变该双位点变异。Objective To analyze the mutation of HBV X region nucleotide sequence in patients with HBV reinfection after liver transplantation. Methods In this study 320 patients received liver transplantation due to HBV-related end stage liver diseases between June 2002 and Dec 2003. Postoperatively polymerase chain reaction was used to amplify their serum HBV DNA fragments for direct sequence analysis. Patients that were followed-up for 1.5 - 3 years were enlisted for analysis. Results All the 11 reinfection recipients showed nucleotide mutations in X region ranging from 5 to 39 sites after transplantation. An A to T mutation at nt1762 and G to A mutation at nt1764 were found in 6 cases. The mutations at nucleotide （nt）1636 - 1741 were found in all 11 cases. Conclusions The results indicated that mutations of nt 1762 and nt 1764 are very common and immunosuppressants cannot change the mutations in patients with HBV reinfection after liver transplantation.

关 键 词：肝移植 肝炎病毒 乙型 核苷酸类 序列分析 再感染 

分 类 号：R686[医药卫生—骨科学]