Myostatin通过Smad3下调MyoD的表达来抑制骨骼肌卫星细胞的分化  

Myostatin Inhibits Myogenic Satellite Cell Differentiation Through Down-regulating MyoD Expression by Smad3

作　　者：阮井玲[1] 甄鑫[1] 刘娣 周佳勃[2] 

机构地区：[1]东北农业大学动物科技学院,哈尔滨150030 [2]东北农业大学,哈尔滨150030

出　　处：《中国生物工程杂志》2008年第5期99-103,共5页China Biotechnology

基　　金：黑龙江省科技攻关项目(GB01B104)

摘　　要：Myostatin基因,是肌肉生长的负调控因子,通过下调MyoD的表达抑制骨骼肌细胞的分化,但具体机制目前尚未完全清楚。以体外培养的猪骨骼肌卫星细胞为实验材料,利用RNA i技术,以Smad3为靶基因进行干扰研究,研究干扰前后猪骨骼肌卫星细胞增殖情况的变化以及MyoD、Myostatin基因的表达规律,进一步阐述3个基因间的调控关系。结果表明,Myostatin通过下调MyoD的表达,抑制骨骼肌卫星细胞的分化,但这种抑制作用是受Smad3调节的。Myostatin is a negative regulator of myogenesis, which inhibits myogenic cell differentiation by down-regulating MyoD expression, but the specific mechanisim is not yet completely clear. The myogenic satellite cells were cultured in vitro. Smad3 was studied by RNAi as the target gene. The influences of Smad3 gene interference on myogenic satellite cell proliferation and the changes of MyoD and Myostatin gene after smad3 gene interference in myogenic satellite cell were researched, and the relationship of regulation among three genes was further explained. Together, the results show that myostatin inhibits myogenic cell differentiation by down-regulating MyoD expression, but this inhibition is mediated through Smad3.

关 键 词：MYOSTATIN SMAD3 MYOD RNAi基因网络 

分 类 号：Q786[生物学—分子生物学]