逆转录病毒表达系统及其在外源蛋白高效表达中的应用  

Retroviral Expression System and Its Application in Efficient Foreign Gene Expression

作　　者：许建[1] 李世崇[1] 陈昭烈[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京100071

出　　处：《中国生物工程杂志》2008年第5期116-121,共6页China Biotechnology

摘　　要：逆转录病毒表达系统是一种新的重组蛋白高效表达系统,它由逆转录病毒载体,包膜蛋白载体和包装细胞系构成,在基因治疗和生物制药领域有很大的应用潜力。逆转录病毒和宿主细胞基因组的重组倾向于发生在转录活性区;口炎疱疹病毒-G蛋白(vesicular stomatitis virus G,VSV-G)能有效扩大逆转录病毒感染宿主细胞的范围、提高逆转录病毒的感染效率;用高滴度的重组病毒感染细胞,经过简单的筛选即可获得高表达细胞株。就逆转录病毒表达系统的组成、感染的特点和机制及其应用前景作了概述。Retroviral expression system which consists of retroviral vector, envelop protein vector and packaging cell line is an efficient expression system for recombinant protein. It has great potential in gene therapy and biopharmacy. Transcriptional active genome regions are the preferred targets for retrovirus integration. Furthermore, VSV-G protein enables this system a broader host range and makes virus integration more efficient. After infection of high -titer virus, high production clones can be selected through simple screening. So far, the research on retrovirus expression system has developed into application in bio-pharmacy industry. Here the composition of this system and the mechanism of virus transduction and summarize the application and prospect of retroviral expression system are introduced.

关 键 词：逆转录病毒表达系统 口炎疱疹病毒-G蛋白 病毒滴度重组蛋白 高效表达 

分 类 号：Q786[生物学—分子生物学]