核转录因子κB在中药金叶败毒制剂抗人巨细胞病毒感染中的作用  

The role of NF-κB in the inhibitory effects of Chinese medicine JinyeBaidu(JYBD) on human cytomegalovirus infection

作　　者：汪辉[1] 李天[1] 凌霞珍[1] 闻良珍[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院妇产科,武汉430030

出　　处：《中国优生与遗传杂志》2008年第5期18-19,17,共3页Chinese Journal of Birth Health & Heredity

基　　金：国家"九五"重点攻关项目资助(96-904-06-08)

摘　　要：目的研究核转录因子NF-κB在金叶败毒制剂(简称中药)抗人巨细胞病毒(HCMV)感染中的作用。方法分别应用原位杂交和半定量RT-PCR方法检测中药和更昔洛韦(GCV)干预HCMV感染的细胞NF-κBp65mRNA及HCMV晚期mRNA的表达水平,观察两种药物对HCMV感染致人胚胎成纤维细胞(HEL)病变(CPE)的影响。结果两种药物对HCMV晚期mRNA的表达和HCMV致细胞病变CPE均有抑制作用,但中药能显著抑制NF-κBp65mRNA的水平,而GCV对此影响不明显。结论中药可能通过抑制核转录因子NF-κB而拮抗HCMV感染,NF-κB信号通路激活可能为HCMV致病机制之一。Objective：To explore the the role of NF-κB in the inhibitory effects of Chinese medicine JinyeBaidu（JYBDG） on human cytomegalovirus infection.Methods：The expressions of NF-κB p65 mRNA and HCMV late mRNA were investigated by means of in situ hybridization and reverse tanscript polymerase chain reaction（RT-PCR） before and after treated by JYBDG or Ganciclovir,separately,and the development of cytopathic effect（CPE） was also observed.Results：Both JYBDG and Ganciclovir could inhibit HCMV late mRNA and CPE.JYBDG had obvious inhibitory effect on expression of NF-κB p65 mRNA,but no effect in the Ganciclovir group.Conclusion：JYBDG has an antiviral efficacy through inhibiting NF-κB activation,NF-κB may play an important role in the mechanism of HCMV deseases.

关 键 词：金叶败毒制剂 巨细胞病毒 核转录因子NF-ΚB 

分 类 号：R285[医药卫生—中药学]