Cdc42在小鼠卵母细胞发育中与纺锤体定位的关系  

Relationship of Cdc42 and spindle positioning during mouse oocyte development

作　　者：梁彬[1] 程大也[2] 

机构地区：[1]中国医科大学高等职业技术学院 [2]中国医科大学附属第一医院输血科,沈阳110001

出　　处：《中国优生与遗传杂志》2008年第5期110-112,共3页Chinese Journal of Birth Health & Heredity

基　　金：教育部博士点基金资助(No20050159023)

摘　　要：目的研究Cdc42与小鼠卵母细胞发育过程中纺锤体定位的关系。方法以小鼠卵母细胞为模型,利用特异性抑制Cdc42活性的Cdc42T17N(GDP结合形式Cdc42),显微注射小鼠卵母细胞。共聚焦显微镜下观察Cdc42T17N mRNA注射组和空质粒注射对照组小鼠卵母细胞发育过程中纺锤体的定位,对结果进行统计分析。结果与对照组小鼠卵母细胞相比,Cdc42T17N mRNA注射组小鼠卵母细胞发育过程中纺锤体的定位分布有所改变。Cdc42T17N mRNA注射组的小鼠卵母细胞,纺锤体一端与卵母细胞皮质层接触百分率下降,与卵母细胞皮质层平行接触百分率上升,差异有统计学意义(P<0.05)。结论Cdc42可能参与小鼠卵母细胞发育过程中纺锤体的定位。Objective：Study the relationship of spindle positioning and Cdc42 during mouse oocyte development.Methods：Mouse oocytes as a model,specific Cdc42 activity inhibitor-Cdc42T17N（GDP-Cdc42） was microinjected into mouse oocytes.Observe spindle positioning between Cdc42T17N mRNA injection group and PCS2 plasmid injection group during mouse oocyte development.Compare the difference of two groups.Results：Compared with the controls,there were changes between Cdc42T17N mRNA injection group and PCS2 plasmid injection group during mouse oocyte development.The percentage of spindle that one pole attached to the cortex decreased（P〈0.05）,and the percentage of spindle juxtaposing the oocyte cortex increased（P〈0.05）.The difference was significant.Conclusion：These results support the contention that Cdc42 involved in mouse oocyte development by interfering with spindle positioning.

关 键 词：CDC42 卵母细胞 纺锤体 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]