银杏叶提取物对溶血卵磷脂胆碱致血管内皮细胞损伤的保护作用  

PROTECTIVE EFFECTS OF EXTRACT OF GINKGO BILOBA (EGb) ON LYSOPHOSPHATIDYLCHOLINE INDUCED DAMAGES OF VASCULAR ENDOTHELIAL CELLS IN VITRO

作　　者：张依宁[1,2,3] 张健[1,2,3] 黄桂秋 顾成云[1,2,3] 陈维洲 

机构地区：[1]上海第二医科大学病理生理学教研室 [2]上海职工医学院 [3]中国科学院上海药物研究所

出　　处：《药学学报》1997年第10期735-739,共5页Acta Pharmaceutica Sinica

摘　　要：应用传代培养的小牛主动脉内皮细胞（ＢＡＥＣ）为实验模型，观察银杏叶提取物（ＥＧｂ）对溶血卵磷脂胆碱（ＬＰＣ）损伤ＢＡＥＣ及其分泌功能的影响。结果发现培养ＢＡＥＣ与ＬＰＣ（２５μｇ·ｍｌ－１）孵育２４ｈ后，出现细胞皱缩成团，大多细胞脱落、甚至细胞解体。细胞内漏出ＬＤＨ增加，脂质过氧化物（ＭＤＡ）含量增高，ＳＯＤ活性降低，而ＰＡＩ活性增高。ＬＰＣ与不同浓度ＥＧｂ共孵育２４ｈ，大部分细胞排列规则，形态基本正常，ＬＤＨ明显减少，ＭＤＡ含量下降，ＳＯＤ活性增高及ＰＡＩ活性降低。结果表明ＬＰＣ可致培养血管内皮细胞损伤，而ＥＧｂ可对抗ＬＰＣ所造成的血管内皮细胞损伤，可能通过清除氧自由基、抗脂质过氧化作用和提高ＥＣ产生抗氧化能力。Effects of EGb on cultured bovine aortic endothelial cells (BAEC) damaged by lysophosphatidylcholine (LPC) were investigated. When endothelial cells were incubated with LPC (2 5 μg·ml -1 ) for 24 h, the endothelial cells turned round pyknotic, exfoliated and broken, and the contents of malondialdehyde (MDA), lactate dehydrogenase (LDH) and plasminogen activator inhibitor (PAI) were markedly increased and the superoxide dismutase (SOD) activity was inhibited. Treatment with EGb at concentrations of 125 and 250 μg·ml -1 , caused a reduction in MDA, LDH and PAI contents, while the SOD activity in the medium was increased and the morphologically damaged BAECs were alleviated. These results suggest that EGb afforded protection against BAECs damages induced by LPC and that the protective effect of EGb may be due to anti lipid peroxidation via free radical scavenging activity.

关 键 词：内皮细胞 溶血卵磷脂胆碱 银杏叶 提取物 

分 类 号：R285.5[医药卫生—中药学]