迁地保护黄檗群体的遗传多样性评价  

Genetic diversity evaluation of ex-situ conservation population of Phellodendron amurense detected by AFLP markers

作　　者：闫志峰[1] 张本刚[1] 张昭[1] 

机构地区：[1]中国医学科学院中国协和医科大学药用植物研究所,北京100094

出　　处：《中国中药杂志》2008年第10期1121-1125,共5页China Journal of Chinese Materia Medica

基　　金：国家中医药管理局科学技术研究专项(04-05ZP07);北京市教委重点学科共建项目(XK100230448)

摘　　要：目的:评价迁地保护黄檗群体的遗传多样性水平。方法:利用8对荧光引物,对10个迁地保护黄檗群体共计67株个体进行AFLP分析。结果:共扩增1 574条谱带,其中多态带1 354条;黄檗物种水平的遗传多样性高于迁地保护群体水平,多态位点百分率PPL分别是86.02,41.55,有效等位基因数Ne分别为1.427 5,1.237 6,Nei基因多样性指数H分别是0.231 2,0.140 0,Shannon信息指数I分别是0.397 3,0.211 3。结论:初步评价了黄檗迁地保护效果。Objective： To evaluate the genetic diversity from ex-situ conservation population of Phellodendron amurense. Method： Genetic diversity in 67 individuals from ex-situ conservation population of P. amurense were assessed using AFLP technique by eight fluorescent labeled primer groups. Result： The eight primer combinations generated a total of 1 574 bands, of which 1 354 were polymorphic. As for P. amurense at species level, the percentage of polymorphic loci （PPL） was 86.02, the effective number of alleles per locus （Ne） was 1. 427 5, the Nei＇s gene diversity （H） was 0.231 2, and the Shannon＇s information index （I） was 0. 397 3. At the population level, the estimates PPL =41.55, H =0. 140 0, Ne =0. 237 6 and I =0. 211 3. Conclusion： The effect of present ex-situ conservation was t）reliminarilv assessed.

关 键 词：珍稀濒危药用植物 黄檗 迁地保护 AFLP 遗传多样性 

分 类 号：R282.5[医药卫生—中药学]