麝香醒脑宁对脑缺血再灌注损伤的保护作用及机制  

Protective effects of Shexiang Xingnaonin on focal cerebral ischemia/reperfusion injury and mechanism

作　　者：李维祖[1] 尹艳艳[1] 曹曦[1] 李卫平[1] 

机构地区：[1]安徽医科大学药理教研室,安徽合肥230032

出　　处：《中国中药杂志》2008年第10期1195-1199,共5页China Journal of Chinese Materia Medica

摘　　要：目的:探讨麝香醒脑宁(Shexiang Xingnaonin,SXN)对脑缺血再灌注损伤的保护作用及其机制。方法:采用大鼠线栓法制备脑缺血再灌注模型,观察SXN对神经功能评分、脑组织病理形态学变化的影响,电镜观察脑组织超微结构,免疫组化检测肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)的表达,血栓形成仪测定体外血栓形成及比值法测定血小板聚集;体外法观察SXN对血凝块的溶解作用。结果:与模型组比较,SXN(0.04,0.08,0.16 g.kg-1)可降低脑缺血再灌注8 h和22 h的神经功能评分,改善脑组织病理形态学和超微结构,抑制脑组织TNF-α的表达;SXN(0.08,0.16 g.kg-1)对体外血栓形成和血小板聚集有一定的抑制作用;SXN(0.16,0.32 g.L-1)在体外对血凝块有较好的溶解作用。结论:SXN对脑缺血再灌注损伤具有一定的保护作用,其机制可能与抑制TNF-α的表达、抑制血栓形成等有关。Objective： To study the protective effects of Shexiang Xingnaonin （SXN） on focal cerebral ischemia/reperfusion injury and its mechanism. Method： Middle cerebral artery occlusion （MCAO） was used to make focal cerebral ischemia/reperfusion model by in travascular nylon filament occlusion. The protective effects of SXN at different doses were evaluated by investigating neuro- logical function score, pathomorphology of brain, the ultrastructure of neuron, expression of tumor necrosis factor-a, thrombogenesis in vitro, platelet aggregation and lysing effect of blood clot in vitro. Result： Compared with model group, SXN （0. 08,0. 16 g·kg^-1） could decrease the neurological score, improve pathomorphology and neuron ultrastructure of brain, inhibit the expression of TNF-a, decrease the length, wet weight and dry weight of thromb and inhibit platelet aggregation. And SXN （0. 16, 0. 32 g·L^-1） could dissolve blood clot in vitro. Conclusion： SXN has protective effects on focal cerebral ischemia/reperfusion injury. The role of inhibit the expression of TNF-a, inhibit thrombogenesis and platelet aggregation might contribute to its neuroprotective effects.

关 键 词：脑缺血再灌注损伤 肿瘤坏死因子-A 血栓形成 

分 类 号：R285.5[医药卫生—中药学]