助孕胶囊对多囊卵巢综合征大鼠卵巢局部细胞因子表达的影响  

Effect of Zhuyun Capsule on Expression of Ovarian Cytokine of Polycystic Ovary Syndrome Rats

作　　者：刘艳巧[1] 党慧敏[1] 刘润侠[1] 康晓玲[1] 陈梅[1] 

机构地区：[1]西安交通大学医学院第二附属医院中西医结合科

出　　处：《中医杂志》2008年第5期453-455,458,共4页Journal of Traditional Chinese Medicine

基　　金：陕西省中医药管理局资助项目(2003-156)

摘　　要：目的观察助孕胶囊对多囊卵巢综合征(PCOS)大鼠血清性激素水平及卵巢局部细胞因子表达的影响。方法采用孕激素联合人绒毛膜促性腺激素(HCG)法制备PCOS模型大鼠。SD大鼠40只随机分为正常对照组、模型组、助孕胶囊组、克罗米酚组,每组10只,运用放射免疫法测定血清性激素水平的变化;免疫组化法检测卵巢组织肿瘤坏死因子α(TNF-α)、胰岛素样生长因子1(IGF-1)、白血病抑制因子(LIF)表达水平并分析各因子之间的关系。结果助孕胶囊可明显降低PCOS大鼠血清睾酮(T)、促黄体生成素(LH)水平,并下调卵巢组织TNF-α和IGF-1的表达,上调卵巢组织LIF的表达。结论助孕胶囊能够通过调整内分泌及影响卵巢局部细胞因子的表达,调整PCOS大鼠性腺功能,促进卵泡的发育成熟及排卵。Objective To observe the effect of Zhuyun Capsule （Pregnant-promoting Capsule） on serum sex hormone and expression of ovarian cytokine of polycystic ovary syndrome （PCOS） rats. Methods Progestogen plus human chorionic gonadotrophin （HCG） was adopted to make PCOS rat models. Totally 40 SD rats were randomized into control group, PCOS model group, Zhuyun Capsule group, and clomiphene citrate group with 10 in each. Radioimmune assay （RIA） was used to measure the sex hormone and immunohistochemical test was done to measure the expression of tumor necrosis factor-α （TNF-α） of ovarian tissue, insulin-like growth factors-1 （IGF-1）, and leukemia inhibitory factor （LIF） to analyze the relations among these factors. Results Zhuyun Capsule could reduce greatly serum testosterone （T） and luteinizing hormone （LH）, decrease expression of TNF-α and IGF-1, but increase expression of LIF. Conclusion Zhuyun Capsule could regulate endocrine function and expression of ovarian cytokine to improve gonad functions of PCOS rats and to promote the maturation of follicle and ovulation.

关 键 词：多囊卵巢综合征 助孕胶囊 大鼠 卵巢 细胞因子 性激素 

分 类 号：R285.5[医药卫生—中药学]