海昆肾喜对阿霉素肾病大鼠肾组织内皮素1及其mRNA表达和肾小球系膜细胞增殖的影响  

Effect of Haikun Shenxi Capsule on Endothelin-1 and mRNA Expression in Kidney Tissue of Rats with Adriamycin-induced Nephrosis and Proliferation of Glomerular Mesanglal Cells

作　　者：刘昆[1] 赵宗江[1] 张新雪[1] 杨美娟[1] 

机构地区：[1]北京中医药大学细胞与生化实验室

出　　处：《中医杂志》2008年第5期456-458,共3页Journal of Traditional Chinese Medicine

基　　金：教育部"长江学者和创新团队发展计划"资助项目(IRT0413);国家自然科学基金重点(30130220);高等学校学科创新引智计划资助项目(B07007);北京市中医管理局"51510"资助项目(JJ2007-006)

摘　　要：目的探讨海昆肾喜对阿霉素肾病大鼠内皮素1及其mRNA表达的影响以及对体外培养肾小球系膜细胞增殖的影响。方法将大鼠随机分为6组、正常组、假手术组、模型组、苯那普利组、海昆肾小剂量组及海昆肾喜大剂量组,10周后采用免疫组织化学及原位杂交方法分析肾组织内皮素1蛋白及mRNA表达情况;以苯那普利为实验对照组,采用MTT比色法测定海昆肾喜对肾小球系膜细胞增殖的影响。结果模型组大鼠肾组织中内皮素1及mRNA表达强阳性;海昆肾喜大、小剂量组较模型组表达显著减少(P<0.05或P<0.01);并且海昆肾喜可以明显抑制肾小球系膜细胞的增殖。结论海昆肾喜能够减少阿霉素肾病大鼠肾组织中内皮素1及mRNA的表达,抑制肾小球系膜细胞的增殖,从而延缓肾衰竭的的病程进展。Objective To study the effect of Haikun Shenxi Capsule（HSC） on endothelin-1 （ET-1） and mRNA expression of rats with adriamycin-induced nephrosis and proliferation of glomerular mesangial cells （GMCs） cultured in vitro. Methods The rats were randomized into 6 groups： normal group, sham operation group, model group, benazepril group, HSC low-and high-dose groups. Ten weeks after treatment, ET-1 and mRNA expression were measured with immunohistochemical and hybridization in situ method. Tak- ing the Benazepril group as the control group, the proliferation of GMCs was detected with MTT method. Results In the model group, ET-1 and mRNA expression presented a strong positive, compared with which, that of the HSC low- and high-dose groups was remarkably reduced （P^0.05）, and the effect of high-dose group was better as the proliferation of GMCs was remarkably suppressed. Conclusion HSC reduces remarkably ET-1 and mRNA expression in kidney tissue of rats with adriamycin-induced nephrosis and decreases the proliferation of GMCs, slowing down the development of renal failure.

关 键 词：海昆肾喜 阿霉素肾病 大鼠 内皮素1 细胞增殖 

分 类 号：R285[医药卫生—中药学]