雷公藤内酯醇对结肠癌细胞COX-2和iNOS表达的抑制作用  

Triptolide Inhibit the Expression of Cyclooxygenase-2(COX-2) and Inducible Nitric Oxide Synthase(iNOS) on Human Colon Cancer Cells

作　　者：夏旭芬[1] 王伟[1] 鲍亚萍[1] 张伟敏[1] 姚航平[2] 童向民[2] 

机构地区：[1]浙江省立同德医院,杭州310012 [2]浙江大学医学院附属第一医院,杭州310003

出　　处：《中国药学杂志》2008年第10期758-761,共4页Chinese Pharmaceutical Journal

基　　金：浙江省卫生厅科研基金资助项目(2006B041);浙江省中医药管理局科研基金资助项目(2005C066)

摘　　要：目的研究雷公藤内酯醇(TP)抑制结肠癌SW114细胞株环氧化酶-2(COX-2)、诱导型一氧化氮合酶(iNOS)及其产物PGE2和NO的表达,探讨TP的抗肿瘤机制。方法不同浓度的TP作用于结肠癌细胞株24h,通过RT-PCR,Western印迹杂交,ELISA检测环氧化酶-2(COX-2)、诱导型一氧化氮合酶(iNOS)及其产物,同时提取各组细胞蛋白质,TransAM测定核转录因子NF-κB活性。结果TP对结肠癌细胞株COX-2和iNOS的表达及其产物PGE2和NO的合成有抑制作用,而且也抑制SW114细胞的NF-κB活性,这些作用与剂量呈依赖关系。结论TP对SW114细胞COX-2和iNOS的表达及其产物PGE2和NO的合成有抑制作用,这种作用可能通过增强TP对核转录因子NF-κB活性的抑制作用而实现,揭示了TP抗肿瘤的部分依据。OBJECTIVE To explore the effects of triptolide （TP） on cell expressions of cyclooxygenase-2 （COX-2） , inducible nitric oxide synthase （iNOS） and their inducing products of PGE2, NO in human colon cancer （SW114） cells, and to study the mechanisms of TP in tumor therapy. METHODS The expressions of COX-2 and iNOS mRNA were analyzed by semi-quantitative RT- PCR. The expressions of COX-2 and iNOS protein were estimated by Western-blot method. The productions of PGE2 and NO in culture supernatants of cell lines were detected with ELISA. NF-κB activity in SW114 cells was measured by an ELISA-based method. RESULTS Triptolide down-regulated COX-2 and iNOS mRNA protein expressions, and inhibited the production of PGE2 and NO. NF-κB activity was suppressed by TP. This effect was highly positive correlated with TP concentrations. CONCLUSION These data suggest that TP maybe reveal the new antitumor mechanism and can be used as a potential chemotherapeutic agent for cancer treatment.

关 键 词：雷公藤内酯醇 结肠癌 环氧化酶-2 诱导型一氧化氮合酶 

分 类 号：R965[医药卫生—药理学]