胰岛素对肾脏近曲小管上皮细胞多巴胺D5受体表达和功能的影响  

Effect of Insulin on D_5 Dopamine Receptor Expression and Function in Renal Proximal Tubule Cells

作　　者：杨剑[1,2] 韩愈[1] 黄河飞[1] 何多芬[1] 糜漫天[2] 曾春雨[1] 

机构地区：[1]第三军医大学大坪医院(野战外科研究所)心内科心血管病研究室,重庆400042 [2]第三军医大学营养与食品卫生学教研室,重庆400038

出　　处：《中华高血压杂志》2008年第5期431-434,共4页Chinese Journal of Hypertension

基　　金：国家自然基金资助项目(30470728、30672199);国家科技 部973计划前期(2008CB517308)

摘　　要：目的观察胰岛素对。肾脏近曲小管上皮(RPT)细胞多巴胺 D_5受体表达与功能的影响。方法以RPT 细胞与多巴胺 D_5受体转染的 HEK293(HEK-D_5)细胞为研究对象,观察胰岛素对 D_5受体表达与功能的影响;并在 Wistar-Kyoto(WKY)大鼠与自发性高血压大鼠(SHR)的 RPT 细胞上比较这种影响的区别。观察在胰岛素[10^(-7)mmol/(L·24 h)]预先作用与否的情况下,D_5受体(D_1类激动剂 Fenoldopam)对 Na^+-K^+-ATP 酶活性的影响。结果胰岛素可升高 WKY 的 RPT 细胞 D_5受体表达,该作用呈现作用浓度与时间依赖性;在 SHR 的 RPT 细胞却出现截然不同的现象,刺激胰岛素受体却降低了 D_5受体的表达;基础状态下,SHR 的 RPT 细胞 D_5受体的表达明显低于 WKY 细胞。与单用 Fenoldopam 相比,在胰岛素(10^(-7)mmol/L/24 h)预先刺激的情况下,Fenoldopam降低 Na^+-K^+ATP 酶的作用增强。结论胰岛素可调节 RPT 细胞 D_5的表达和功能,该调节作用异常可能在高血压的发生发展中发挥一定作用。Objective To investigate the effect of insulin on D5 dopamine receptor expression and function in renal proximal tubule （RPT）. Methods Immortalized RPT cells and D5 receptor transfected HEK293 （ HEK-D5 ） cells were used in the study to investigate the effect of insulin on D5 receptor expression and function, and those effects were compared in RPT cells from Wistar-Kyoto （WKY） rats and spontaneously hypertensive rats （SHR）. The function of D5 receptor was determined by measurement of the Na^＋-K^＋-ATPase activity in HEK-D5 cells. Results Insulin increased D5 receptor protein expression in a concentration and time-dependent manner in WKY RPT cells, but not in SHR. The basal level of D5 receptor expression was higher in WKY cells than that in SHR cells. Stimulation with fenoldopam（ D1-like dopamine receptor agonist） inhibited the Na^＋-K^＋-ATPase activity; pretreatment with insulin increased the inhibitory effect of fenoldopam on Na^＋-K^＋-ATPase activity in HEK-D5 cells. Conclusion The abnormal regulation of insulin on D5 receptor expression and function might be involved in the pathogenesis of essential hypertension.

关 键 词：多巴胺D5受体 胰岛素 肾小管上皮细胞 高血压 

分 类 号：R544.1[医药卫生—心血管疾病]