干细胞因子对糖尿病小鼠结肠Cajal间质细胞的干预效应  

Effect of stem cell factor on colon interstitial cells of Cajal in murine with diabetes mellitus

作　　者：徐丽明[1] 林琳[1] 汤玉蓉[1] 张红杰[1] 李学良[1] 

机构地区：[1]南京医科大学第一附属医院消化内科,江苏省南京市210029

出　　处：《世界华人消化杂志》2008年第12期1294-1298,共5页World Chinese Journal of Digestology

基　　金：国家重点基础研究发展计划资助项目;No.2006CB503908~~

摘　　要：目的:探讨外源性干细胞因子(stem cell factor,SCF)能否改善糖尿病(diabetes mellitus,DM)小鼠结肠Cajal间质细胞(interstitial cells of Cajal,ICC)的异常病变.方法:DM小鼠一次性ip链脲佐菌素(STZ,150mg/kg)造模,将♂C57/BL6小鼠分为正常对照组(control组)、糖尿病组(DM组)、糖尿病+外源性SCF组(DM+SCF组);DM+SCF组ipSCF0.2μg/(kg·d),control组和DM组每天ip等量的磷酸盐缓冲液(pH=7.4),干预6wk后处死所有小鼠,以Westernblot检测远端结肠组织中SCF的表达情况,以免疫组化、透射电镜和Westernblot观察远端结肠ICC的变化.结果:DM小鼠远端结肠组织中SCF水平明显降低(178.97±13.51vs200.25±16.48,P<0.05),且伴结肠组织中ICC数量减少(72±10vs102±12,P<0.05)、超微结构严重破坏.DM鼠给予外源性SCF干预后,结肠组织中SCF表达(210.14±11.8)上调(P<0.05),且ICC的数量(87±10,P<0.05)和超微结构显著改善.结论:外源性SCF对糖尿病相关的结肠ICC异常病变有一定改善或逆转作用.AIM： To investigated whether exogenous stem cell factor （SCF） can improve the diabetesassociated depletion of interstitial cells of Cajal （ICC） in mice with diabetes mellitus （DM）. METHODS： DM mice were intraperitoneally injected with streptozocin （STZ） to induce an experimental model. Male C57/BL6 mice were randomly divided into control group, DM group and DM ＋ SCF group. The mice in DM ＋ SCF group were given exogenous SCF （0.2μg/kg per day, ip） for 6 wk, and the mice in control group and DM group were given the same amount of phosphate buffer （pH = 7.4）. All the mice were sacrificed after 6 wk. ICC changes in the distal colon were assessed by immunohistochemistry, transmission electron microscopy and Western blot, and SCF expression in the distal colon was analyzed by Western blot. RESULTS： The expression of SCF in the distal colon was significantly reduced in DM group as compared with that in the control group （178.97±13.51 vs 200.25±16.48, P 〈 0.05）, accompanied with the depletion （72±10 vs 102±12, P 〈 0.05） and microscopic lesions of ICC in the distal colon. The expression of SCF in the distal colon was increased in DM ＋ SCF group （210.14±11.8, P 〈 0.05）, along with the dramatic improvement of ICC quantity （87±10, P 〈 0.05） and ultrastructure in the distal colon as compared with those in DM group. CONCLUSION： Exogenous SCF may improve the DM-associated depletion of colon ICC.

关 键 词：糖尿病 干细胞因子 CAJAL间质细胞 结肠 免疫印迹 免疫组化 

分 类 号：R587.2[医药卫生—内分泌] R574[医药卫生—内科学]