8Hz次声对大鼠学习记忆能力和神经元再生的影响  

The effect of 8Hz infrasound on the learning and memory ability and proliferation in rats′ brain

作　　者：袁华[1] 龙华[2] 牟翔[1] 李玲[3] 张美霞[1] 葛雪松[1] 刘静[1] 瞿丽莉[1] 陈景藻[1] 

机构地区：[1]第四军医大学西京医院康复与理疗科,西安710032 [2]第四军医大学唐都医院骨科 [3]北京中国人民解放军总医院第一附属医院康复理疗科

出　　处：《中国康复医学杂志》2008年第5期385-387,I0001,共4页Chinese Journal of Rehabilitation Medicine

基　　金：全军医学科学技术研究“十五”计划指令性课题(OlL071)

摘　　要：目的:观察次声作用对大鼠学习记忆能力和脑内神经元再生的影响。方法:将36只SD大鼠随机分为对照组和8Hz130dB次声作用组。次声作用组大鼠暴露于8Hz130dB次声,分别作用1、2和4周;对照组大鼠除无次声作用外,余处理均同于次声作用组。各组大鼠实验结束后进行水迷宫测评后取脑,抗Ki-67免疫组化显示脑内脑室下带(subventricular zone,SVZ)和海马齿状回颗粒层下增生带(subgranular proliferative zone,SGZ)中神经细胞再生情况的变化。结果:8Hz130dB次声作用从第2周开始增生的神经细胞数目开始减少,到第4周时增生期的神经细胞数目比对照组大鼠减少,同时大鼠表现出水迷宫测评中找到站台的潜伏期延长。结论:8Hz130dB次声慢性作用后大鼠引起脑内尤其海马SGZ中增生的神经细胞数目减少,这可能是次声作用引起学习记忆功能障碍的原因之一。Objective： To study the effect of 8Hz infrasound with different duration （1, 2 and 4 weeks） on the proliferation in rats＇ brain. Method：Thirty-six SD rats were randomly divided into 1, 2 and 4 weeks infrasound exposure groups and control group （n=12）. Infrasound exposure groups were exposed to 8Hz 130dB infrasound, 2h/d for 1, 2 and 4 weeks respectively. The control rats were treated as well as the infrasound exposure group except for the infrasound exposure. At the last 3 days of the treatment duration, rats were applied for Morris water maze test. After then their brains were removed and anti-Ki67 immunohistochemical staining was used to check the proliferation in rats＇ brain. Result： After 2 weeks exposure to 8Hz 130dB infrasound, the performance in water maze began to be impaired. The Ki-67 positive cells in SGZ decreased the top decrease occurred in 4th week. Conclusion： 8Hz 130dB infrasound could down regulate the proliferation in the SGZ, which may be involved in the impairment of learning and memory.

关 键 词：次声 脑室下带 海马齿状回颗粒层下增生带 学习记忆能力 增生 

分 类 号：R594[医药卫生—内科学]