运动训练对大鼠缺血下肢血管新生的影响  

Effects of hindlimb ischemia and exercises training on microangium in rats with ischemic limbs

作　　者：葛红卫[1] 何延政[2] 梅燕[2] 刘勇[2] 曾宏[2] 施森[2] 文雪刚[2] 

机构地区：[1]苏州大学附属第三医院血管外科,213003 [2]泸州医学院附属医院血管外科,646000

出　　处：《中国康复医学杂志》2008年第5期413-415,共3页Chinese Journal of Rehabilitation Medicine

摘　　要：目的:观察下肢缺血及运动训练对大鼠缺血下肢微血管生成的影响。方法:SD大鼠30只随机分为A、B、C3组各10只,A、B组建立大鼠下肢缺血模型,C组仅作皮肤切开缝合。建模1周后A组大鼠跑步训练(30min/d)。B、C组日常活动,运动训练4周后处死大鼠,取大腿内收肌组织块免疫组化检测微血管密度(MVD)、血管内皮生长因子(VEGF)、碱性成纤维细胞生长因子(bFGF)表达,比较3组差异。结果:肌组织MVD、VEGF、bFGF免疫组化均显示:A组高于B组(P<0.01),B组高于C组(P<0.01);A、B、C组间差异均存在显著性。结论:下肢缺血刺激可以促进血管新生、成血管因子表达增加,运动训练可以增强该效应。Objective：To observe the effects of hindlimb ischemia and exercises training on microangium in rats with ischemic limbs.Method：Thirty health male Sprague-Dawley rats were divided into experiment group（A）,control group （B） and pseudosurgery group （C） randomly. Hindlimb ischemia was established in rats （A and B） by surgical excision of right femoral arteries.Rats in group A were trained to run 30min once a day at the standard for 4 weeks,then all the rats were sacrificed to obtain the adductor of the ischemic upper leg and fix. MVD,VEGF, bFGF in tissues were measured by immunohistochemistry to compare the difference among 3 groups. Result：MVD, VEGF,bFGF in tissues measured by immuno-histochemistry techniques displayed as：group A〉group B （P〈0.01）;group B 〉 group C （P〈0.01）,significant differences were among group A,B and C.Conclusion：Lower limb ischemia can increase the angiogenesis and exercises training can enhance this effect.

关 键 词：缺血 运动训练 血管新生 

分 类 号：R87[医药卫生—运动医学]