内生真菌ZJUF0986代谢的抗菌活性物质特性  

The antifungal activity of the bioactive compound produced by endophytic fungus ZJUF0986

作　　者：王国平[1] 郑必强[1] 章初龙[1] 林福呈[1] 

机构地区：[1]浙江大学生物技术研究所

出　　处：《植物保护学报》2008年第2期127-131,共5页Journal of Plant Protection

基　　金：浙江省重大科技攻关项目(2006C12008)

摘　　要：内生真菌紫杉木霉ZJUF0986菌株产生的活性代谢产物Ⅰ对植物病原真菌具有广谱抑菌活性。通过紫外吸收、薄层色谱、捷克八溶剂系统纸色谱、pH纸色谱及不同pH和温度处理手段,研究了该活性代谢产物Ⅰ的部分理化性质。结果表明,活性代谢产物Ⅰ的最大紫外吸收峰为199nm;以石油醚:乙酸乙酯=95:5为展开剂薄层层析时,样品斑点的Rf值为0.58;捷克八溶剂系统纸色谱和pH纸色谱图谱与已知各类抗生素纸图谱相比较,活性代谢产物Ⅰ是一类低极性、弱酸性、非水溶性的抗生素;活性代谢产物Ⅰ在酸性至弱碱性和低于100℃的条件下相当稳定,在pH>10和121℃下活性物质不稳定。Bioactive metabolite Ⅰ produced by endophytic fungus Trichoderma taxi ZJUF0986 displays broad antifungal activity to many phytopathogenic fungi. The partial physical and chemical properties of the bioactive metabolite Ⅰ was analyzed by ultraviolet absorbability, thin-layer chromatography, paper chromatography pH and Doskochilova system, and treatment with different pH and temperature levels. Results showed that the maximum UV absorb wavelength was 199 nm. The R：f value of the metabolite Ⅰ was 0.58 when thin-layer chromatography in petroleum ether： ethyl acetate （95 ： 5 ） as mobile phase. The comparison of paper chromatography pH and Doskochilova system between the metabolite Ⅰ and various known antibiotics indicated that the metabolite Ⅰ was a low polar, weakly acerbic and hydrophobic antibiotic. And the metabolite Ⅰ maintained stability at acidic to alkalescent and low than 100 ℃ conditions, it becomes unstable at pH 〉 10 or 121℃.

关 键 词：内生真菌 紫杉木霉 代谢产物 理化性质 

分 类 号：S476[农业科学—农业昆虫与害虫防治]