^103钯放射性支架对bcl-2表达的影响和意义的研究  

Expression and significance of bcl-2 gene in apoptotic muscle cells ^103Pd radioactive stent bile duct in dogs

作　　者：何贵金[1] 鞠光 于发强[1] 高沁怡[3] 许书河[4] 高红[5] 姜维国[6] 蒋涛[5] 戴显伟[1] 马凯[1] 

机构地区：[1]中国医科大学附属二院普外科,沈阳110004 [2]沈阳市新城子区医院外科 [3]中国医科大学附属一院同位素室 [4]中国原子能研究院同位素研究室,北京100050 [5]中国医科大学附属二院儿外实验室 [6]中国医科大学第二临床学院病理科

出　　处：《中华肝胆外科杂志》2008年第4期255-257,共3页Chinese Journal of Hepatobiliary Surgery

基　　金：辽宁省自然科学基金资助项目（20042067）;辽宁省教育厅基金（SL045）;辽宁省计划课题基金（20062250） 许书河 高红 姜维国 蒋涛 戴显伟 马凯

摘　　要：目的为探讨γ射线对犬bcl-2基因的影响及意义。方法将^103钯（^103Pd）放射性支架和普通支架分别植入犬的肝外胆管，免疫组化方法检测γ射线诱导的犬胆管壁凋亡增殖平滑肌细胞中的bcl-2表达，计数凋亡细胞，计算机图像分析系统检测两组胆管管腔面积。结果bcl-2表达^103Pd支架组较普通支架组为弱，胆管出现增殖平滑肌细胞明显凋亡；肝外胆管无明显狭窄；普通支架组未出现增殖平滑肌细胞明显凋亡，肝外胆管有明显狭窄。结论bcl-2表达水平与细胞凋亡发生和细胞对辐射敏感性有关；^103Pd放射性支架通过降低bcl-2表达，促进胆管增殖平滑肌细胞凋亡，从而抑制肝外胆管狭窄。Objective To study the expression and significance of bcl-2 gene in the apoptotic muscle cells in γ-radiation-induced muscle cell lines. Methods bcl-2 gene in the control and r-radiation-induced apoptotic muscle cells was analyzed by using immunohistochemical technique. Results The bcl-2 gene transcript more highly expressed in general stent dog bile duct than in ^103Pd radioactive stent dog bile duct, but apoptotic muscle cells were more highly in ^103 Pd radioactive stent dog bile duct than in general stent dog bile duct. Conclusion The low level of expression of bcl-2 gene may aid the effects to understand the muscle cell sensitivity to γ-radiation apoptosis. ^103Pd radioactive stent may reduce the expression of bcl-2 gene in dog bile duct and prevent the biliary narrowing after dog bile duct injury by balloon.

关 键 词：细胞凋亡 ^103Pd支架 Γ射线 BCL-2基因 

分 类 号：R686[医药卫生—骨科学]