大鼠肝移植肝动脉重建方法改进的实验研究  

Modified rearterialization in rat liver transplantation

作　　者：孙强[1] 朱晓峰[1] 何晓顺[1] 马毅[1] 陈细桃[2] 黄洁夫[1] 

机构地区：[1]中山大学附属第一医院器官移植中心,广州510080 [2]中山大学附属第一医院动物实验中心,广州510080

出　　处：《中华肝胆外科杂志》2008年第4期269-271,共3页Chinese Journal of Hepatobiliary Surgery

基　　金：本课题受卫生部临床重点学科项目（项目编号2001321）;广东省重大科技联合攻关项目（项目编号2002830207）;China Medical Boardin New York（项目编号06837）资助

摘　　要：目的构建重建肝动脉血供的大鼠肝移植模型。方法在经典“二袖套”法的基础上，利用24G静脉留置针外鞘管制作动脉支架管吻合供体腹腔动脉及受体肝总动脉。结果共进行肝移植26次。所有手术均单人操作完成。手术成功率（存活24h以上）为92．3％（24／26）。动脉吻合时间仅需（1．70±0．46）min。经解剖发现术后第1天及第30天所有动物肝动脉均通畅，搏动良好，各肝叶入口处动脉亦充盈良好。未发现胆道坏死，胰腺坏死或由于周围组织包绕形成的胆道内漏等。结论利用支架法吻合供体腹腔动脉及受体肝总动脉的方法是一种操作简便、耗时短、对正常生理干扰小、通畅率高的理想的大鼠肝移植肝动脉重建方法。Objective To establish a new method for hepatic arterial reconstruction in rat liver transplantation. Methods Based on the＇two cull＇method, celiac artery of donor and the common hepatic artery of recipient were anastomosed with a splint which is made of intraventous catheter 24 G. All animals were killed to evaluate occlusion rates of the reconstructed arteries 1 d and 30 d after transplantation. Result A total of 26 cases of rat liver transplantation were performed by one person. The survival rate （survival time 〉24 h） was 92.3 % （24/26）. The time expenditure to finish arterial anas tomosis was only 1.70±0. 46 min. The arteries of all animals were patent ld and 30d after reperfusion. There was no necrosis of bile duct and pancreas, bile leakage and internal fistulae. Conclusion Anastomosing celiac artery of donor and the common hepatic artery of recipient by a splint is an ideal method of rearterialization in rat liver transplantation because of its simplicity, convenience and high rate of perfusion.

关 键 词：肝移植 大鼠 肝动脉 

分 类 号：R686[医药卫生—骨科学]