分光光度法测定核黄素碘盐碘含量方法的研究  

Study on determination of iodine content in riboflavin-iodinated salt by spectrophotometry

作　　者：马有香 焦淑婷[2] 张金生 柴清波 

机构地区：[1]河北省邯郸市峰峰矿区疾病预防控制中心,河北邯郸056200 [2]河北省邯郸市疾病预防控制中心,河北邯郸056002

出　　处：《中国卫生检验杂志》2008年第3期460-461,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的：建立一种用分光光度法测定核黄素碘盐碘含量的方法。方法：在酸性条件下，加入的次氯酸钠将样品中的碘离子氧化成碘酸根，草酸除去多余的次氯酸钠，碘酸根氧化碘化钾析出碘，使溶液呈现黄色，以样品基体色作参比，从而排除核黄素干挠。结果：用分光光度法测定核黄素碘盐，碘含量在0-160μg呈线性关系，相关系数为0.9997，相对标准偏差在1.63%-2.77%，回收率为89.5%-101.2%，最低检出限为0.62μg。若取10.0 g样品，则最低检出浓度为0.25 mg/kg。结论：与国标氧化还原滴定法比较，分光光度法测定核黄素碘盐碘含量，排除了由于核黄素本身颜色的影响而出现的干扰，具有准确、快速等优点。Objective： A method for the determination of iodine content in riboflavin - iodinated salt was studied, Methods： oxidized by NaClO in acidic solution, iodine - ion could turn to iodine then 12 could be extracted under KI oxidized condition, solution emerged yellow, iodine content couldn＇t interfere by riboflavin. Results：Beer＇s law was obeyed in the range of 0 - 160 μg, relational coefficient being r = 0. 9997, The relative standard deviation was in the range of 1.63% - 2.77%. The recovery was 89. 5% - 101. 2%, The detection limit was 0. 62 μg.The lowest detection concentration was 0. 25 mg/kg, Conclusion： Compare with Oxidation- reduction titration method, the determination of iodine content couldn＇t interfered by riboflavin. The method is simple and accurate and it is suitable for determining iodine content.

关 键 词：核黄素碘盐 分光光度法 碘含量 

分 类 号：O657.32[理学—分析化学]