骨灵片对去卵巢骨质疏松大鼠腰椎骨形态计量学及生物力学的影响  

Effects of Chinese herbal medicine invigorating the kidney(Guling Pian) on histomorphology and biomechanical of lumbar vertebra in ovariectomized rats with osteoporosis

作　　者：张娟[1] 李娟[1] 黄少慧[1] 吴湘慧[1] 

机构地区：[1]南方医科大学附属南方医院中医科,广东广州510515

出　　处：《中国老年学杂志》2008年第9期833-835,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助项目(30371840)

摘　　要：目的观察补肾中药——骨灵片对去卵巢(OVX)骨质疏松(OP)大鼠腰椎的骨组织形态计量学以及生物力学影响,进一步探讨补肾中药治疗OP的机制。方法选择3月龄雌性SD大鼠36只,随机分为假手术组、OVX组、骨灵片低、中、高剂量组和雌激素组,每组各6只。试验4个月后采用骨形态计量学检测腰椎骨的动静态参数,生物力学凹入法检测腰椎的最大载荷和刚度。结果①与OVX组比较,骨灵片各剂量组和雌激素组的骨小梁分离度、骨形成率(BFR/BS、BFR/BV)、每mm破骨细胞数均有显著下降(P<0.01);骨灵片中剂量组BFR/TV和中高剂量组的骨矿化沉积率(MAR)减少有显著差异(P<0.01);骨灵片中高剂量组和雌激素组骨小梁面积百分数、厚度和数量显著升高且中剂量组的骨小梁厚度比雌激素组有显著增加(P<0.05);②生物力学上,与去卵巢组比较,雌激素组和骨灵片各剂量组的刚度以及骨灵片中高剂量组的最大载荷均有显著升高(P<0.01)。骨灵片高剂量组在最大载荷上的提高较雌激素组有显著差异(P<0.05)。结论补肾中药骨灵片具有促进骨形成和抑制骨吸收的双重作用,降低骨转换,使骨结构得到改善,并明显增加骨生物力学强度。Objective To observe the histomorphology effects and biomechanical abilities of Chinese herbal medicine invigorating the kidney （Guling Pian） on osteoporotic （OP） ovariectomized （OVX） rats model,and explore its therapeutic mechanism. Methods 36 femal 3-month-old SD rats were randomized into sham-operated, OVX, Guling Pian low, medium, high dosage and ethynylestradiol groups, 6 in each group. The dynamic and static parameters of lumbar vertebrae were tested by bone morphometry, the maximum load and rigidity of lumbar vertebrae were determined by biomechanical indentation testing 4 months later. Results （1）The bone trabecula resolution, bone formation rate （BFR）/BS and BFR/BV,the number of osteoclast per mm in different dosages of Guling Plan and ethynylestradiol groups were significantly decreased than those in OVX group （ P 〈 0. 01 ）. The BFK/TV in moderate dosage Guling Plan group and bone mineral apposition rate （MAR） in middle and high dosages of Guling Plan groups were significantly decreased （ P 〈 0. 01 ）. The bone trabecula area percentage, thickness and number in moderate and high dosages of Guling Pian and ethynylestradiol groups obviously increased, the bone trabecula thickness of moderate dosage of Guling Plan group was higher than that in ethynylestradiol group （ P 〈 0. 05 ）. （2）The rigidity of ethynylestradiol and different dosages of Guling Plan groups and the maximum load in moderate and high dosages of Guling Plan groups were significantly higher than those in OVX group （P 〈0. 01）. The maximum load of high dosage of Guling Pian group was significantly different from that of ethynylestradiol group （ P 〈 0. 05 ）. Conclusions Guling Pian has double effects on restraining bone absorption and promoting bone formation, may decrease bone turnover to improve bone quality and to increase bone biomechanics intensity.

关 键 词：补肾中药 骨质疏松 骨组织形态计量学 生物力学 

分 类 号：R289.5[医药卫生—方剂学]