Jurkat细胞增殖过程中S期激酶相关蛋白2与p27^kip1的表达变化及意义  

Expression variation and significance of Skp2 and p27^kip1 during the proliferation of Jurkat cells

作　　者：陆建新[1] 王熵婵[1] 沈爱国[1] 赵玥铭[1] 孙承龙[2] 张冬梅[1] 程纯[1] 

机构地区：[1]南通大学微生物与免疫学教研室,226001 [2]南通大学附属医院输血科

出　　处：《中华肿瘤杂志》2008年第5期330-334,共5页Chinese Journal of Oncology

基　　金：基金项目：国家自然科学基金资助项目（30300099）;江苏省自然科学基金资助项目（BK2003035）;江苏省卫生科研项目（H200632）;江苏省高校自然科学研究项目（04KJB320114）;江苏省高等学校研究生创新计划项目（xm04-66）

摘　　要：目的探讨S期激酶相关蛋白2（skp2）与p27^kip1在淋巴瘤细胞株Jurkat细胞增殖过程中的表达变化及意义。方法采用免疫沉淀法检测Jurkat细胞中p27^kip1与Skp2的结合情况；采用血清饥饿合并释放法同步化处理Jurkat细胞，再分别用Western blot技术、免疫荧光双标技术及核浆分离法检测p27^kip1和Skp2在Jurkat细胞中的表达变化及亚细胞定位。结果p27^kip1与Skp2能够相互结合。在Jurkat细胞增殖过程中，p27““蛋白表达减少，其中在细胞核内的减少更明显；Skp2蛋白表达增加，其中在细胞浆中的增加更明显。结论在Jurkat细胞增殖过程中，Skp2在细胞浆中的合成增加，并可能通过加快p27^kip1的泛素化降解使细胞核内p27^kip1显著减少，从而推动细胞周期的进程。Objective To investigate the expression variation and significance of Skp2 and p27^kip1 during the proliferation of lymphoma cell line Jurkat cells. Methods The binding of p27^kip1and Skp2 in Jurkat cells were detected by immunoprecipitation. Jurkat cells were treated with serum starvation and release synchronization. The expression variation and subcellular localization of p27^kip1 and Skp2 were detected by subcellular fractionation, Western blot and double immunofluorescence labelling. Results The results of immunoprecipitation suggested that p27^kip1 and Skp2 could bind each other in Jurkat cells. During the proliferation of Jurkat cells, the protein expression of p27^kip1 decreased and intranuclear p27kipl decreased significantly, while the Skp2 protein increased and cytoplasmic Skp2 increased significantly. Com^lusion During the proliferation of Jurkat cells, the increased cytoplasmic synthesis of Skp2 may speed up p27^kip1 degradation via the ubiquitin-proteasome pathway, then intranuclear p27^kip1 decreases significantly, leading to an increased cell cycling activity.

关 键 词：JURKAT细胞株 P27^KIP1 SKP2 

分 类 号：R686[医药卫生—骨科学]