生存素及bcl-2基因表达在吲哚美辛抗胃癌细胞株MGC803增殖、诱导凋亡中的作用  

The effects of Survivin、bcl-2 mRNA expression in indomethacin inhibiting proliferation and inducing apoptosis on gastric cancer cell lines MGC803

作　　者：袁伟建[1] 卢笛[1] 牛小平[1] 张桂英[1] 石科[2] 

机构地区：[1]中南大学湘雅医院消化内科,湖南长沙410008 [2]中南大学湘雅医院检验科,湖南长沙410008

出　　处：《中国医院药学杂志》2008年第9期686-689,共4页Chinese Journal of Hospital Pharmacy

基　　金：湖南省卫生厅科研基金项目(编号:B2004-033)

摘　　要：目的:探讨吲哚美辛(IN)对胃癌细胞株MGC803增殖、凋亡的影响及可能的机制。方法:观察不同浓度IN(50,100,200μmoL·L-1)对MGC803细胞增殖、凋亡及抗凋亡基因生存素、bcl-2 mRNA表达的影响,MTT法检测细胞生长抑制率;荧光染色检测细胞凋亡率;流式细胞仪测定细胞周期变化;RT-PCR检测生存素、bcl-2 mRNA的表达。结果:IN对MGC803细胞具有抑制增殖、促进凋亡的作用,并具有浓度、时间依赖性;IN作用于细胞48h后,细胞周期改变表现为G0/G1期比例增加,S期及G2/M期下降;细胞经200μmoL·L-1IN作用后,生存素mRNA表达逐渐减弱(P<0.05),bcl-2 mRNA表达无明显变化。结论:IN可抑制MGC803细胞增殖、诱导凋亡,干扰细胞增殖周期,生存素mRNA表达下调可能是其作用机制之一。OBJECTIVE To investigate the effects of Survivin. bcl-2 mRNA expression in indomethacin （IN） inhibiting proliferation and inducing apoptosis on gastric cancer cell lines MGC803. METHODS MGC803 cells were treated by IN （50,100, 200 μmol·L^-1）. The proliferation was detected by MTT assay and apoptosis was measured by fluorescence staining after the cells were treated by IN for 12,24,48,72 hours. Cell cycle was detected by flow cytometry after the cells were treated by IN for 48 hours. Survivin and bcl-2 mRNA expression of the cells treated by IN （200 μmol·L^-1 ） were detected by RT-PCR. RESULTS As the concentration increasing and time elongation, the effects of IN inhibiting proliferation and inducing apoptosis on MGC803 cells reinforced. G0/G1 phase was increased and S and G2/M phase was decreased after the cells were treated by IN. The Survivin mRNA expression decreased, but the bcl-2 mRNA expression did not alter after treated by IN. CONCLUSION IN could arrest the cell cycle, inhibit proliferation, induce apoptosis on MGC803 cell and decreased Survivin mRNA expression.

关 键 词：吲哚美辛 胃癌细胞株 增殖 凋亡 生存素 bcl-2 

分 类 号：R96[医药卫生—药理学]