何首乌的18S rRNA基因序列分析  

Analysis of 18S rRNA Gene Sequence of Polygonum multiflorum

作　　者：严萍[1] 焦旭雯[1] 庞启华[2] 赵树进[3] 

机构地区：[1]华南理工大学生物科学与工程学院,广东广州510006 [2]华南师范大学生命科学学院,广东广州510631 [3]广州军区广州总医院,广东广州510010

出　　处：《华南理工大学学报（自然科学版）》2008年第4期151-154,共4页Journal of South China University of Technology(Natural Science Edition)

基　　金：广东省自然科学基金资助项目(6104397)

摘　　要：为探讨野生和种植品种物种间的亲缘关系并为其鉴别提供分子依据,分析了不同产地野生何首乌Polygonum multiflorumThunb及种植品种和伪品芭蕉的核基因组18S rRNA序列.何首乌的18S rRNA基因序列长度为1 809 bp,且高度保守,在680、1 712和1 724位点存在碱基替代.与何首乌基因序列相比较,伪品芭蕉有71个位点发生碱基置换,在284和1 537位点上分别插入了C和A碱基,而在135和500位点分别缺失了T和A.通过18S rRNA基因序列的同源性分析,基本可以认为野生品种和种植品种基原一致,说明DNA测序技术是一种准确而有效的何首乌分子鉴定方法.In order to reveal the relationship between the cultivated and the wild Polygonum multifiorum from different geographical locations and to provide evidences for the molecular identification, the 18S rRNA gene sequences of wild Polygonum multiflorum Thunb, the cultivated Polygonum multiflorum and Musa Basjoo Sieb. ＆ Zucc were analyzed. The results show that the 18S rRNA gene sequences of Polygonum multiflorum are 1809bp in length and are highly conserved, and that nucleotide substitutions occur at positions 680, 1712 and 1724. As compared with Polygonum multijflorum sequences, Musa Basjoo Sieb. ＆Zucc is of 71 nucleotide substitutions, a T＆A deletion at positions 135 and 500 and a C＆A insertion at positions 284 and 1 537. According to the homology of 18S rRNA gene sequences, the cultivated and the wild Polygonum multiflorum have identical botanical origins, which means that DNA sequencing is an accurate multiflorum. and effective method of molecular identification of Polygonum

关 键 词：分子鉴定 何首乌 基因 序列分析 

分 类 号：Q94[生物学—植物学]