北柴胡快速繁殖及种子萌发条件研究  

Rapid propagation and seed germinating condition of Bupleurum chinense

作　　者：郝建平[1] 徐笑飞[1] 杨东方[1] 侯晶[1] 胡侃[1] 秦雪梅[2] 

机构地区：[1]山西大学生命科学与技术学院,山西太原030006 [2]山西大学化学化工学院药学系,山西太原030006

出　　处：《中草药》2008年第5期752-756,共5页Chinese Traditional and Herbal Drugs

基　　金：山西省科技攻关项目(051077-2)

摘　　要：目的 建立北柴胡的选优育优技术体系，规模化生产北柴胡优质种苗和种子。方法 应用植物组织培养技术分别对种植于山西陵川、万荣及甘肃陇西、陕西商洛等8个地区的优质北柴胡进行快速繁殖；对种植于山西万荣的中柴1号北柴胡种子进行种皮损伤和植物激素处理，比较各种处理对种子萌发率的影响。结果 附加6-BA 1．0mg／L和KT 0．2mg／L的B5培养基适合于各地北柴胡芽的快速增殖与生长；附加NAA 0．1mg／L、IBA0．5mg／L和DSC1．0mg／L的1／2MS培养基对北柴胡试管苗的生根有良好的促进作用；北柴胡试管苗的年繁殖系数超过1×10^8，移栽成活率达94％～97％。植物激素处理对北柴胡种子萌发无明显作用，打磨损伤处理种皮可使种子的萌发率提高20％，并且能大幅度缩短种子的萌发时间。结论植物组织培养技术是快速、大量繁殖优质北柴胡的有效手段；一定程度的种皮损伤可以促进北柴胡种子的萌发。Objective For selecting and developing the excellent Bupleurum chinense to mass-produceseedlings and seeds in high quality. Methods B. chinense was picked from eight different areas, such as Lingchuan and Wanrong county in Shanxi Province, Longxi county in Gansu Province, and Shangluo in Shaanxi Province, etc. Rapid propagation was done. The testa of Zhongchai No. 1 was scrapped or seedswere soaked in different phytohormones. The effects on germination rates of seeds were compared. Results The optimum medium for bud propagation was B5 supplemented with 1.0 mg/L 6-BA and 0.2 mg/L KT. The optimum medium for root induction of test-tube plantlets was 1/2 MS added with 0.1 mg/L NAA,0.5 mg/L IBA, and 1.0 mg/L DSC. The annual propagation coefficient of B. chinense plants was more than 1×10^8, and the survival rate of transplantation reached to 94%—97%. The phytohormone has little effect on seed germination of B. chinense, but scraping the testa could increase the germination percentage of seeds to 20% and shorten the germinating time greatly. ConcLusion By tissue culture of excellent B. chinense, a great deal of plants and seeds could be produced in short time. By scrapping testa in a certain extent, the germination of seeds could be increased.

关 键 词：北柴胡 快速繁殖 种子萌发 药用植物 种质资源 

分 类 号：R282.21[医药卫生—中药学]