绞股蓝叶片分化与植株再生  

Blade differentiation and plantlet regeneration of Gynostemma pentaphyllum

作　　者：赵瑞强[1] 凌征柱[2] 罗育[1] 李雄英[1] 蒋军富[1] 吴耀生[1] 

机构地区：[1]广西医科大学,广西南宁530021 [2]广西药用植物园,广西南宁530023

出　　处：《中草药》2008年第5期757-759,共3页Chinese Traditional and Herbal Drugs

基　　金：广西区自然科学基金(桂科基0575065);中科院植物生理生态研究所开放课题

摘　　要：目的建立高效稳定的绞股蓝受体系统,为绞股蓝基因转化研究奠定基础。方法以五叶绞股蓝Gynostemma pentaphyllum叶片作为外植体,采用不同配比激素的MS培养基进行诱导培养获得丛生芽,进而生根获得再生植株。结果采用MS+6-BA 1.0 mg/L培养基时,绞股蓝叶片分化频率最高(40%),MS+6-BA1.0 mg/L适合绞股蓝丛生芽继代增殖,1/2 MS适宜诱导生根获得健全再生植株。结论为利用根癌农杆菌介导法进行绞股蓝基因转化建立了稳定的直接分化再生系统。Objective To construct a stably-performing and high-efficiency regeneration system of Gynostemma pentaphyllum and lay a foundation for the gene transformation of it. Methods The blades of five-leaf G. pentaphyllum were used as the explants and cultured in MS media with different portions ofhormone to induce fascicled-bud, root and plantlet regeneration. Results The medium suitable for inducing the blade differentiation of G. pentaphyllurn was MS＋6-BA 1.0 mg/L and for the frequency ofblade differentiation could reach 40%. The cultural medium MS＋6-BA 1.0 mg/L was suitable for the sub-multiplication of fascicled-bud and the medium 1/2 MS for root inducement and the plantletregeneration. Conclusion A stably-performing acceptor system of the direct differentiation for Agrobacterium tumefaciens mediated gene transformation of G. pentaphyllum blades is constructed.

关 键 词：绞股蓝 叶片分化 植株再生 

分 类 号：R282.21[医药卫生—中药学]