掌叶大黄细胞悬浮系统和根培养系统对鬼臼毒素的生物转化研究  

Biotransformation of podophyllotoxin by cell suspension and root culture systems of Rheum palmatum

作　　者：杨世海[1] 刘家源[2] 于博 刘晓峰[4] 

机构地区：[1]吉林农业大学中药材学院,吉林长春130118 [2]哈尔滨商业大学药学院,黑龙江哈尔滨150080 [3]华禾药业股份有限公司,北京100035 [4]吉林亚泰(集团)股份有限公司,吉林长春130031

出　　处：《中草药》2008年第5期763-766,共4页Chinese Traditional and Herbal Drugs

摘　　要：目的对鬼臼毒素的生物转化进行研究。方法利用掌叶大黄细胞悬浮系统和根培养系统进行生物转化。采用色谱技术和波谱手段对转化产物进行分离鉴定。结果加入鬼臼毒素对掌叶大黄细胞悬浮系统和根培养系统的pH值无明显影响;两种组培系统均能转化鬼臼毒素。在本实验条件下,细胞悬浮系统使73.8%的鬼臼毒素发生了转化,根培养系统中使56.3%的鬼臼毒素发生了转化。两种系统对鬼臼毒素的转化产物不同,前者只转化生成鬼臼苦素,后者转化生成表鬼臼毒素等多种化合物。结论掌叶大黄细胞悬浮系统和根培养系统均能有效转化鬼臼毒素。Objective To investigate the biotransformation of podophyllotoxin by cell suspensionculture and root culture systems of Rheum palmatum. Methods Plant tissue culture technology was employed. The products were isolated by silica gel column chromatography and their structures wereelucidated by spectral methods. Results Adding of podophyllotoxin had no obvious effects on the pH value of cell suspension culture and root culture systems of R. palmatum. In the case of present study, 73.8% of podophyllotoxin was converted by cell suspension culture and 56.3% by root culture. Theproducts were different between the two culture system. Podophyllotoxin was converted into picropodophyllin with cell suspension culture system and many other products besides epipodophyllotoxin with root culture system. Conclusion Both of cell suspension culture and root culture systems of R. palmatum are able to convert podophyllotoxin.

关 键 词：掌叶大黄 细胞悬浮系统 根培养系统 鬼臼毒素 鬼臼苦素 表鬼臼毒素 生物转化 

分 类 号：R282.6[医药卫生—中药学]