何首乌和夜交藤药材指纹图谱研究与评价  

Fingerprints for Radix Polygoni Multiflori and Caulis et Folium Polygoni Multiflori

作　　者：苏建[1] 袁志芳[1] 吴一兵[2] 张兰桐[1] 王春英[1] 

机构地区：[1]河北医科大学药学院药物分析教研室,河北石家庄050017 [2]河北医科大学药学院新药开发教研室,河北石家庄050017

出　　处：《中草药》2008年第5期766-769,共4页Chinese Traditional and Herbal Drugs

基　　金：河北省自然科学基金资助项目:何首乌降血脂作用的药效物质基础研究(C2006000791)

摘　　要：目的建立何首乌、夜交藤药材指纹图谱分析方法,并依据指纹图谱对两种药材进行比较,为科学评价与有效控制何首乌、夜交藤药材质量提供新方法。方法何首乌和夜交藤分别用甲醇和醋酸乙酯超声处理,提取液用HPLC进行测定。HPLC法采用C18色谱柱,乙腈-0.1%磷酸梯度洗脱,检测波长分别为265和290 nm,体积流量1mL/min,柱温30℃。结果建立了何首乌、夜交藤药材HPLC指纹图谱,并对不同产地何首乌、夜交藤药材进行了相似度比较。测定结果显示不同产地药材存在较大差异,化学成分组成及质量分数存在一定差异。结论所建立的方法简便、可靠,可用于不同产地何首乌、夜交藤药材的指纹图谱测定和质量评价,为全面有效控制何首乌药材的内在质量提供了依据。Objective To establish HPLC fingerprints for Radix Polygoni Multiflori and Caulis etFolium Polygoni Multiflori, to compare the fingerprints of the above two crude drugs collected from different habitats, and to provide a new method for scientific evaluation and quality control of them.Methods Radix Polygoni Multiflori and Caulis et Folium Polygoni Multiflori were extracted by supersonic wave with methanol and ethyl acetate. The chromatographic procedure for fingerprints was carried out using G18 as an analytic column, gradient eluted with a mixture consisting of acetonitrile and 0. 1% H3PO4. Results This experiment established a specific method for the quality control of Radix Polygoni Multiflori and Caulis et Folium Polygoni Multiflori, and compared the similarity of Radix Polygoni Multiflori with Caulis Polygoni Multiflori from different habitats. Conclusion This method is very simple, reliable, and can be used for the fingerprint assay and quality control of Radix PolygoniMultiflori and Caulis et Folium Polygoni Multiflori. It provides a new method as a comprehensive quality control item for Radix Polygoni Multi flori and Caulfs et Folium Polygonf Multi flori.

关 键 词：何首乌 夜交藤 高效液相色谱法 指纹图谱 

分 类 号：R282.7[医药卫生—中药学]