诱导子对海洋真菌Penicillium crustosum 2A4-Z15菌株生物碱代谢的调节作用  

Effect of elicitors on alkaloid metabolism of a marine fungus Penicillium crustosum 2A4-Z15

作　　者：边猛[1] 田黎[1] 张久明[1] 郑立[2] 周俊英[1] 崔志松[2] 林文翰[3] 

机构地区：[1]青岛科技大学生物系,山东青岛266042 [2]国家海洋局第一海洋研究所现代化学分析室,山东青岛266061 [3]北京大学医学部,北京100083

出　　处：《海洋学报》2008年第2期117-123,共7页

基　　金：国家“863计划”(2007AA09Z435,2006DFA31100,2007AA091507);国家自然科学基金(40776098,20602009)

摘　　要：分别采用4种生物源诱导子和5种非生物源诱导子,对具有较强抑菌活性的海洋真菌壳青霉Penicillium crustosum2A4-Z15菌株进行诱导,考察诱导子对于菌株产生物碱的代谢调节作用。结果表明,不同来源的诱导子对菌株的生物碱产量和生物量影响不同,生物源诱导子的正向调节作用较明显,且其中以细菌源诱导子最为明显。当海洋细菌源诱导子在2A4-Z15菌株发酵对数生长期末,以10μg/cm3的浓度加入时,发酵物生物碱产量最高,较对照提高44.1%。A marine fungus Penicillium crustosum 2A4- Z15, which has strong inhibition activity, was separated from South China Sea and identified. It was treated by four biotic elicitors and five abiotic elicitors. Four biotic elicitors originated from different marine microorganism including Bacillus sp. , Penicillium sp. , Trichorderma sp. and Fucarium sp.. Five abiotic elicitors included three amino acids used as precursors and two inhibitors. The effect of different elicitors on alkaloid metabolism of the marine fungus was studied. The results show that different elicitors have different effect on alkaloid metabolism and biomass. Generally speaking, biotic elicitors have evidently positive influence on them. Amino acids added as precursors can show positive influence only with proper kind and concentration. Inhibitors tested both show negative influence. Alkaloid metabolism can be enhanced by 44. 1% by the marine bacterial elicitor with a 10μg/cm^3 concentration added at logarithmic period.

关 键 词：海洋真菌 诱导子 生物碱代谢 

分 类 号：Q939.5[生物学—微生物学]