甘薯茎线虫rDNA-ITS1区的PCR扩增与序列分析  

PCR and sequence analyse of rDNA-ITS1 region of sweet potato stem nematode

作　　者：章淑玲[1] 张绍升[1] 

机构地区：[1]福建农林大学植物保护学院,福州350002

出　　处：《植物病理学报》2008年第2期132-135,共4页Acta Phytopathologica Sinica

基　　金：国家自然科学基金资助项目(30370931)

摘　　要：利用PCR技术获得了甘薯茎线虫rDNA-ITS1区序列。序列分析表明,采自我国河北、山东、安徽的甘薯茎线虫16个地理种群的ITS1区序列分化为短型（S）和长型（L）2种基因型。山东费县芍药山乡4个地理种群为L型,ITS1区长度为466 bp;河北、安徽和山东费县新庄乡的12个地理种群为S型,ITS1区长度为288 bp。甘薯茎线虫与鳞球茎茎线虫（Di-tylenchus dipsaci）的rDNA-ITS1序列同源性为52.0%-52.5%,与腐烂茎线虫（D.destructor）序列同源性为82.0%-85.4%;我国甘薯茎线虫不同地理种群间的序列同源性为96.6%-100.0%。Sequences of rDNA-ITS1 region of sweet potato stem nematode were obtained by PCR. The result of sequence analyse showed that the sequences of the nematode populations isolated from Hebei, Shandong and Anhui, had two types of genes, type ＂Short＂ （S） and type ＂Long＂ （L）. The rDNA-ITS1 sequences of type ＂S＂ was 288 bp and type ＂L＂ was 466 bp. Type ＂L＂ were the populations from Shaoyao, Fexiang, Shandong province; type ＂S＂ were the nematode populations from Hebei province, Anhui province and Xinzhuang, Fexiang, Shandong province. The rDNA-ITS1 sequences homology were 82.0% - 85. 4% between sweet potato stem nematode and Ditylenchus destructar （Ab363110）, and 52.0% - 52.5% between sweet potato stem nematode and D. dipsaci （AF363110）. The rDNA-ITS1 sequences homology were 96.6% - 100.0% among the geographic populations of sweet potato stem nematode in China.

关 键 词：甘薯茎线虫 rDNA-ITS1 PCR 腐烂茎线虫 

分 类 号：S435.313.4[农业科学—农业昆虫与害虫防治]