大白菜抗芜菁花叶病毒的QTL分析  

Analysis of QTL for Turnip mosaic virus resistance in Chinese cabbage

作　　者：张俊华[1] 屈淑平[1] 崔崇士[1] 

机构地区：[1]东北农业大学,哈尔滨150030

出　　处：《植物病理学报》2008年第2期178-184,共7页Acta Phytopathologica Sinica

基　　金：国家"863"项目(2003AA207120);东北农业大学博士启动基金项目

摘　　要：以大白菜高抗TuMV-C3株系的高代自交系A52-2和感病自交系GCⅣ杂交的F2代255个单株作为构建遗传图谱的作图群体,通过EST-PCR-RFLP和AFLP分子标记的遗传分析,构建了包含12个连锁群,由124个遗传标记组成的大白菜分子遗传图谱。其中包括6个EST-PCR-RFLP多态性位点和118个AFLP多态性位点,该图谱覆盖长度为683.9 cM,平均图距5.52 cM。利用Windows QTL CartographerV 2.0软件和复合区间作图法进行分析,共检测到4个抗TuMV-C3株系的QTLs位点。A population of Chinese cabbage F2 generation including 255 individual plants was derived from the cross between resistance lines A52-2 and susceptible lines GCⅣ. A molecular genetic map of Chinese cabbage was constructed with Mapmaker/EXP 3.0 b software. A total of 124 markers （ 118 AFLP markers and 6 EST-PCR-RFLP markers） were mapped to 12 linkage groups, covering 683.9 cM with an average distance of 5.52 cM between loci. 4 QTLs controlling TuMV-Cz resistance at artificial inoculation were identified with Windows QTL CartographerV 2.0 software and Interval Mapping method.

关 键 词：大白菜 分子遗传图谱 数量性状位点(QTL) 芜菁花叶病毒C3株系 

分 类 号：S436.341[农业科学—农业昆虫与害虫防治]