寄主诱导下的玉米弯孢叶斑病菌致病性分化相关蛋白的动态变化研究  

The dynamic changes of pathogenesis-related proteins of a low virulent Curvularia lunata isolate after reinoculating maize inbreds

作　　者：翟羽红[1] 陈捷[1] 刘力行[1] 黄秀丽[1] 程小舟[2] 朱恒[1] 陈云鹏[1] 徐书法[1] 

机构地区：[1]上海交通大学农业与生物学院,上海200240 [2]山东农业大学植物保护系,泰安271018

出　　处：《植物病理学报》2008年第2期203-207,共5页Acta Phytopathologica Sinica

基　　金：国家重点基础研究发展计划(2006CB101901);国家自然学基金项目(30471057);国家科技支撑项目(2006BAD08A06)

摘　　要：Reinoculation and two-dimensional electrophoresis were performed to analyze the pathogenicity differentiation of Curvularia lunata.Resistant host inbred lines Shen135,Mo17,and 78599-1 were reinoculated(six generations) with low virulent isolate WS18.Results showed that the disease index had no significant change for the first 2 generations of inoculation.At the third generation,the incidence of disease was increased and the number of differential expressed proteins of mycelia were more than that in the first 2 generations.More than 100 differential expressed proteins were found in the mycelia of fifth generation when compared with the original one.In the experiment,10 differentially expressed proteins were identified by MALDI-TOF-MS analysis.Three proteins were related directly to the differentiation of virulence,2 were related to allergen,4 were related to the metabolism of carbon or signaling pathway and 1 was unkonwn to Curvularia lunata.Reinoculation and two-dimensional electrophoresis were performed to analyze the pathogenicity differentiation of Curvularia lunata. Resistant host inbred lines Shen135, Mo17, and 78599-1 were reinoculated （six generations） with low virulent isolate WS18. Results showed that the disease index had no significant change for the first 2 generations of inoculation. At the third generation, the incidence of disease was increased and the number of differential expressed proteins of mycelia were more than that in the first 2 generations. More than 100 differential expressed proteins were found in the mycelia of fifth generation when compared with the original one. In the experiment, 10 differentially expressed proteins were identified by MALDI-TOFMS analysis. Three proteins were related directly to the differentiation of virulence, 2 were related to allergen, 4 were related to the metabolism of carbon or signaling pathway and 1 was unkonwn to Curvularia lunata.

关 键 词：玉米弯孢叶斑病菌 致病性分化 寄主植物 动态变化 相关蛋白 诱导 玉米主产区 寄主选择性 

分 类 号：S432.44[农业科学—植物病理学] S651.034[农业科学—农业昆虫与害虫防治]