基于小麦白粉病菌rDNA ITS序列的PCR分子检测  

PCR detection of Blumeria graminis f.sp.tritici based on the sequences of rDNA ITS

作　　者：曾晓葳[1] 骆勇[2] 周益林[1] 段霞瑜[1] 

机构地区：[1]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100094 [2]中国农业大学植物病理学系,北京100094 [3]美国加州大学Kearney农业研究中心,Parlier CA 93648

出　　处：《植物病理学报》2008年第2期211-214,共4页Acta Phytopathologica Sinica

基　　金：国家自然科学基金项目(30771386);国家科技支撑计划项目(2006BAD08A01和2006BAD08A05);公益性行业科研专项(nyhyzx07-048)

摘　　要：Wheat powdery mildew(Blumeria graminis f.sp.tritici) is the one of main wheat diseases in China.Based on the internal transcribed spacer(ITS) sequences of ribosome of B.graminis f.sp.tritici,three molecular primer pairs(F1/R,F2/R and F3/R) were designed to detect the fungal pathogen of wheat powdery mildew.The species specificity of these primers was confirmed.F1/R was demonstrated a higher sensitivity than the other two primer pairs,and could detect as low as 1 pg DNA of B.graminis f.sp.tritici.Furthermore,F1/R primer pair was used to detect the pathogen DNA extracted from wheat leaves showing chlorosis and typical symptoms of powdery mildew caused by artificial inoculation with B.graminis f.sp.tritici.The preliminary results demonstrated the usefulness of this primer pair and its potential applications in efficient detection of wheat powdery mildew pathogen from leaves with latent infections at early growth stages of wheat.Wheat powdery mildew （Blumeria graminis f. sp. tritici） is the one of main wheat diseases in China. Based on the internal transcribed spacer （ITS） sequences of ribosome of B. graminis f. sp. tritici, three molecular primer pairs （F1/R, F2/R and F3/R） were designed to detect the fungal pathogen of wheat powdery mildew. The species specificity of these primers was confu＇med. F1/R was demonstrated a higher sensitivity than the other two primer pairs, and could detect as low as 1 pg DNA of B. graminis f. sp. tritici. Furthermore, F1/R primer pair was used to detect the pathogen DNA extracted from wheat leaves showing chlorosis and typical symptoms of powdery mildew caused by artificial inoculation with B. graminis f. sp. tritici. The preliminary results demonstrated the usefulness of this primer pair and its potential applications in efficient detection of wheat powdery mildew pathogen from leaves with latent infections at early growth stages of wheat.

关 键 词：小麦白粉病菌 PCR分子检测 ITS序列 RDNA 小麦病害 小麦产量 预测预报 白粉菌 

分 类 号：S435.121.4[农业科学—农业昆虫与害虫防治] S641.303.6[农业科学—植物保护]