中国柑橘黄龙病病原16SrDNA序列研究  

Studies on 16S rDNA Sequence of Citrus Huanglongbing Bacteria in China

作　　者：丁芳[1] 洪霓[1] 钟云[2] 易干军[2] 王国平[1] 

机构地区：[1]华中农业大学植物科学技术学院,武汉430070 [2]广东省农业科学院果树研究所,广州510640

出　　处：《园艺学报》2008年第5期649-654,共6页Acta Horticulturae Sinica

基　　金：国家‘863’计划项目(2006AA10Z434);湖北省科技攻关项目(2006AA203B05);华中农业大学博士科研启动项目(2006XRC057);教育部新教师基金资助项目(20070504079)

摘　　要：运用PCR技术对来自中国7省区不同寄主上的黄龙病病原16SrDNA基因区进行了PCR-RFLP-SSCP分析,采用3种限制性内切酶进行单酶切、双酶切及三酶切反应,对酶切产物进行了单链构象多态性(SSCP)分析。结果表明:来自7省区不同寄主上9个黄龙病病原菌分离物的16SrDNA无可见变异;同时对9个有代表性的分离物16SrDNA进行了克隆测序,序列多重比对结果与PCR-RFLP-SSCP一致,从而在分子水平上证明了中国柑橘黄龙病病原菌的16SrDNA序列高度保守,在不同的地域、寄主内没有发现分子变异,为进一步研究柑橘黄龙病病原菌系统进化奠定了基础。PCR- RFLP-SSCP analysis was carried out to study the 16S rDNA of the citrus Huanglongbing （HLB） bacterial isolates collected from 7 provinces in China. Three restriction endonucleases were used to digest the target DNA fragment of 16S rDNA. The digested products were subjected to single-strand conformation polymorphism （SSCP） analysis. The results showed that there was no obvious difference among the 9 representative isolates from 7 provinces in 16S rDNA. The 16S rDNAs of the 9 representative isolates were cloned and sequenced. The result of the multiple alignment analysis of the sequences was in agreement with that of PCR-RFLP-SSCP analysis. It revealed that the citrus Huanglongbing bacteria isolated from different areas of China were highly conservative in their 16S rDNA sequence, and no molecular change were found among isolates from different hosts and different geographic areas. The result laid a foundation for further research on systematic evolution of HLB bacteria.

关 键 词：柑橘 黄龙病 病原菌 16S RDNA PCR-RFLP-SSCP 克隆 序列分析 

分 类 号：S666[农业科学—果树学]