镍、镉对生物膜损伤机理的FTIR研究  

FTIR Study on the Mechanisms of Nickel and Cadmium-Induced Cell Membrane Injuries

作　　者：姜厚理[1] 宋占军[1] 周晓[2] 刘世杰[2] 江泉观[2] 

机构地区：[1]军事医学科学院国家生物医学分析中心,北京市太平路27号100850 [2]北京医科大学公共卫生学院,北京市学院路100083

出　　处：《光谱实验室》1997年第4期6-9,共4页Chinese Journal of Spectroscopy Laboratory

摘　　要：本文采用傅里叶变换红外（FTIR）漫反射光谱技术，研究比较了镍与镉对人类红细胞膜的联合损伤作用，同时对其作用机制进行了探讨。通过观察分析两金属与红细胞膜作用前后细胞膜红外光谱中1742、1662、1636、1556、1464、1405和1389cm-1等吸收峰的变化，表明两金属离子单独或同时存在均可使膜蛋白质发生变性和不可逆聚合、使膜脂质发生相变以及可能的重金属离子与蛋白质分子中-COOH侧链成盐等分子改变。实验还表明镍、镉对细胞膜的作用机制是类似的，联合作用时两金属离子可能协同引起细胞膜的损伤。Cell membrane is the first toxic target of many xenobiotics and membrane damages ptay an important role in toxic cell injury studies. In this research, structural changes in red blood cell membraneat molecufor level after interaction with nickel and cadmium alone or in combination were investinatedwith fourier-transform inftared diffese spectroscopy. Apparent spectral variances at 1742, 1662,1 636, 1556, 1464, 1405 and 1389 cm-1 were observed, which revealed thedenaturation of membraneproteins, coagulation of lipids and possible stheincation. The results also showed that the injuries ofnickel and cadmium on cell membrane are sAnfor and joint contamination mny cause sgnergistic adverse effects.

关 键 词：FTIR 镍 镉 细胞膜 生物膜损伤 损伤机理 

分 类 号：R992[医药卫生—毒理学] R995[医药卫生—药学]