β-环糊精的双核铜络合物对芳香基氨基酸分子识别的研究  

Studies on the Molecular Recognition Between Copper(Ⅱ) Complex with β Cyclodextrin and Aromatic Aminoacids

作　　者：金瑞祥[1,2] 何锡文[1,2] 

机构地区：[1]天津医科大学药学系 [2]南开大学化学系

出　　处：《分析科学学报》1997年第3期177-181,共5页Journal of Analytical Science

基　　金：国家自然科学基金;国家教委博士点专项科研基金

摘　　要：用荧光分光光度法和紫外分光光度法研究了β－环糊精双核铜络合物（Ｃｕ２－β－ＣＤ）在强碱性溶液中与氨基酸的超分子作用，发现Ｃｕ２－β－ＣＤ与芳香基氨基酸的荧光光谱和紫外光谱发生明显变化，Ｃｕ２－β－ＣＤ对于芳香基氨基酸具有分子识别作用．对于酪氨酸和苯丙氨酸，主客体间形成超分子络合物的化学计量比为１：４．用Ｓｃａｔｃｈａｒｄ方程或Ｌｉｎｅｗｅａｖｅｒ－Ｂｕｒｋ曲线分别求得了它们的物理常数．推测Ｃｕ２－β－ＣＤ与芳香基氨基酸形成超分子络合物的主要作用力是静电力，与β－ＣＤ和氨基酸的疏水作用有所不同．The supramolecular actions between the copper(Ⅱ) complex with β cyclodextrin(Cu 2 β CD) and amino acids in alkaline solution were studied by means of fluorescence and UV spectrophotometry. It is found that the fluorescence spectra and UV spectra of Cu 2 β CD with aromatic amino acids had changed obviously and there was molecular recognition between Cu 2 β CD and aromatic amino acids. The stoichiometric ratio of the supramolecular complex formed form the subject and object was found to be 1∶4 (Cu 2 β CD∶Tyr or Phe). The physical constants were determined using Scatchard equation and Lineweaver Burk curve. The main binding force of the supramolecular complex of Cu 2 β CD with aromatic amino acids was inferred to be static electric force, which was different from the hydrophobic process of β CD with amino acids

关 键 词：双核铜 配合物 氨基酸 超分子 分子识别 Β-CD 

分 类 号：O614.121[理学—无机化学] Q517.02[理学—化学]