Genetic Variation of Host Populations of Liriomyza sativae Blanchard  

作　　者：WANG Li-ping DU Yu-zhout HE Ya-ting ZHENG Fu-shan LU Zi-qiang 

机构地区：[1]Institute of Applied Entomology, Yangzhou University, Yangzhou 225009, P.R.China [2]State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of AgriculturalSciences, Beijing 100094, P.R.China

出　　处：《Agricultural Sciences in China》2008年第5期585-590,共6页中国农业科学（英文版）

基　　金：supported by the grants from the National Natural Science Foundation of China(30370932);the National Basic Research Program of China(2006CB102002);the Opening Foundation of the State Key Laboratory for Biology of Plant Disease and Insect Pests,China.

摘　　要：In this study, partial sequences of the mitochondrial cytochrome oxidase subunit I （mtDNA-COI） gene and the ribosomal internal transcribed spacer 1 （rDNA-ITS 1） gene, isolated from five artificial populations of Liriomyza sativae （Diptera： Agromyzidae）, were sequenced and compared, to analyze their genetic variation. Analysis of the mtDNA-CO1 gene showed that a low genetic variation was detected among the five populations and only five variable sites were found in the nucleotide sequences. Most of the observed variations that occurred within the populations were because of nucleotide transitions, whereas, the interpopulation variation was because of the differences in haplotype frequencies occurring among the host populations. Analysis of the rDNA-ITS1 gene revealed a small diversity in the five host populations. The trend of genetic differentiation in the host populations was consistent with the preference of L. sativae to the plant hosts.In this study, partial sequences of the mitochondrial cytochrome oxidase subunit I （mtDNA-COI） gene and the ribosomal internal transcribed spacer 1 （rDNA-ITS 1） gene, isolated from five artificial populations of Liriomyza sativae （Diptera： Agromyzidae）, were sequenced and compared, to analyze their genetic variation. Analysis of the mtDNA-CO1 gene showed that a low genetic variation was detected among the five populations and only five variable sites were found in the nucleotide sequences. Most of the observed variations that occurred within the populations were because of nucleotide transitions, whereas, the interpopulation variation was because of the differences in haplotype frequencies occurring among the host populations. Analysis of the rDNA-ITS1 gene revealed a small diversity in the five host populations. The trend of genetic differentiation in the host populations was consistent with the preference of L. sativae to the plant hosts.

关 键 词：Liriomyza sativae host populations mtDNA-COI rDNA-ITS 1 genetic differentiation 

分 类 号：S433[农业科学—农业昆虫与害虫防治]