Application of a TLC chemical method to detection of cyclotides in plants  

作　　者：XU WenYan TANG Jun JI ChangJiu HE WenJun TAN NingHua 

机构地区：[1]State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy ofSciences, Kunming 650204, China [2]Graduate University of Chinese Academy of Sciences, Beijing 100049, China

出　　处：《Chinese Science Bulletin》2008年第11期1671-1674,共4页

基　　金：the National Natural Science Foundation of China (Grant No. 30725048);Foundation of the West Light Program of Chinese Academy of Sciences

摘　　要：Iodine, Dragendorff's reagent, ninhydrin and Coomassie brilliant blue G-250 reagents were used to detect cyclotides from proteins, cyclopeptides, linear-peptides and amino acids in the thin layer chromatography (TLC). Cyclotides could be distinguished from the others by comparing the TLC col-oring plots with G-250 and ninhydrin reagents. With this method, cyclotides were discovered in Viola labridorica, V. tricolor, V. hamiltoniana, Momordica charantia, and M. cochinchinensis and over 10 cyclotides were isolated, three of which were determined as known cyclotides cycloviolacin O2, kalata B1 and vary peptide A.Iodine, Dragendorff＇s reagent, ninhydrin and Coomassie brilliant blue G-250 reagents were used to detect cyclotides from proteins, cyclopeptides, linear-peptides and amino acids in the thin layer chromatography （TLC）. Cyclotides could be distinguished from the others by comparing the TLC coloring plots with G-250 and ninhydrin reagents. With this method, cycloUdes were discovered in Viola labridorica, V. tricolor, V. hamiltoniana, Momordica charantia, and M. cochinchinensis and over 10 cyclotides were isolated, three of which were determined as known cyclotides cycloviolacin O2, kalata B1 and vary peptide A.

关 键 词：薄层色谱法 植物生物化学 蛋白质 大环寡肽 

分 类 号：Q946.1[生物学—植物学] O657.7[理学—分析化学]