红细胞悬液传感器的研制  

Studies on Human Erythocyte Electrodc Sensitive to Hydrogen Peroxide

作　　者：赵景婵[1] 李汉杰[1] 郭治安 

机构地区：[1]西北大学化学系电分析化学研究所

出　　处：《分析试验室》1997年第4期9-12,共4页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金

摘　　要：以人红细胞作为过氧化氢酶的来源，用尼龙网共价键合，并与氧电极偶合制得过氧化氢传感器，研究了尼龙网上活化酶的固定化，测试介质等有关实验条件和参数。测得在ｐＨ８的磷酸盐缓冲溶液中，电极响应的线性范围为３×１０－５～３×１０－３ｍｏｌ／Ｌ，测定了传感器在果珍，鱼浆和磷酸盐缓冲溶液中对过氧化氢的回收率，获得满意结果。The human erythrocytes have been immobilized on activated nylon net which is fixed on an oxygen probe.This sensor has been utilized in determining hydrogen peroxide ,some experimental conditions such as activation of nylon net,immobilization of the catalyst of human erythrocytes and the measurement medium have been investigated.The linear response range of the sensor is 3×10 -5 ～3×10 -3 mol/L and the correlation coefficient is 0.9987.The lifetime of the sensor is 70d.Satisfactory recoveries are obtained from fruit juice,fish berry and phosphate buffer.

关 键 词：生物传感器 红细胞悬液 传感器 过氧化氢 

分 类 号：Q811.4[生物学—生物工程]