白假丝酵母菌对人阴道上皮细胞TLR4mRNA表达的影响  

Effects of candida albicans on the expression of Toll-like receptor 4 mRNA in cultured human vaginal epithelial cells

作　　者：张岩[1] 吴文湘[1] 刘朝晖[1] 赵敏[1] 廖秦平[1] 

机构地区：[1]北京大学第一医院妇产科,北京积水潭医院妇产科100034

出　　处：《中国妇产科临床杂志》2008年第3期187-190,I0001,共5页Chinese Journal of Clinical Obstetrics and Gynecology

基　　金：国家“十五”科技攻关计划课题(2004BA720A0501);北京市科技计划项目课题(Y0204003040731)资助

摘　　要：目的探讨白假丝酵母菌对人阴道上皮细胞Toll样受体4(Toll-like receptor4,TLR4)mRNA表达的影响。方法用白假丝酵母菌热灭活孢子刺激体外培养的人阴道上皮细胞12h和24h,应用实时定量逆转录聚合酶链反应(QRT-PCR)检测细胞TLR4mRNA的表达水平。结果正常人阴道上皮组织和体外培养人阴道上皮细胞均表达TLR4mRNA。白假丝酵母菌热灭活孢子刺激12h,上皮细胞中TLR4mRNA表达明显高于对照组和刺激24h组P<0.05,刺激24h组与对照组比较,无明显差异P=0.50。结论人阴道上皮细胞构成性表达少量TLR4,白假丝酵母菌刺激12h时人阴道上皮细胞表达TLR4mRNA增加,推测TLR4可能在人阴道上皮细胞识别白假丝酵母菌和启动防御感染的早期起一定作用。Objective To investigate the effects of candida albicans on the expression of Toll-like receptor 4 （TLR4） mRNA in cultured human vaginal epithelial cells. Methods The primary cultured human vaginal epithelial cells were stimulated with heat - killed Candida albicans blastoconidia for 12 h and 24 h and the expression of TLR4 mRNA was measured by quantitative real-time RT -PCR （QRT-PCR）. Results TLR4 mRNA was minimally expressed in normal human vaginal epithelial tissues and cells. The expression of TLR4 mRNA in cultured human vaginal epithelial cells at 12 h after stimulation with heat- killed candida albicans blastoconidia was significant higher than those at 24 h and the control, respectively （P〈0.05）. There was no statistically significant difference for TLR4 mRNA between vaginal epithelial cells at 24 h stimulated with candida albicans blastoconidia and those not stimulated （P=0.50）. Conclusions Human vaginal epithelial cells both in vivo and in vitro can express constitutively trace amounts of TLR4. Our findings suggest that TLR4 may play a partial role in the recognition of candida albicans and startup host defense mechanism at the early stage of candida albicans infection of human vaginal epithelial cells.

关 键 词：白假丝酵母菌 TOLL样受体4 上皮细胞 阴道 外阴阴道假丝酵母菌病 

分 类 号：R711.3[医药卫生—妇产科学]