阿替洛尔对过氧化氢损伤乳鼠心肌细胞有保护作用  

Protective effects of Atenolol on over-oxidation injury of neonatal SD rat cardiomyocytes

作　　者：戴振华[1] 王亮[1] 雍永宏[1] 盛燕辉[1] 杨荣[1] 孙伟[1] 孔祥清[1] 曹克将[1] 

机构地区：[1]南京医科大学第一附属医院心血管病中心,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2008年第5期618-620,634,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：南京医科大学科技发展基金资助项目(NJ03048)

摘　　要：目的:观察阿替洛尔对过氧化氢(H2O2)致心肌细胞氧化损伤的保护作用,并探讨此作用的机制。方法:用H2O2作用于新生SD大鼠心肌细胞,建立心肌细胞过氧化氢损伤模型。用阿替洛尔预处理后,观察心肌细胞状态,测定心肌细胞存活率、乳酸脱氢酶(LDH)、丙二醛(MDA)活性。结果:阿替洛尔提高心肌细胞过氧化损伤的细胞存活率(P<0.05),减少LDH和MDA的生成(P<0.05)。结论:阿替洛尔对心肌细胞过氧化损伤有一定起保护作用。Objective：To observe protective action of Atenolol on hydrogen peroxide （H2O2） induced cardiomyocytes over-oxidation injury and investigate the mechanism, nethods：A model of cardiomyocytes injuried by H2O2 was established, and the protective effects of Atenolol on the injury was investigated, The morphology of cardiomyocytes and cardiomyocytes surviving fraction were measured. The activities of lactate dehydrogenase（LDH） and malondialdehyde（MDA） were also assayed. Results：Atenolol dose enhance cardiomyocytes surviving fraction （P 〈 0,05）, and decrease the activities of LDH and MDA in the injuried myocytes （P 〈 0.05）. Conclusion：Atenolol has the protective effects on cardiomyocytes injured by H2O2 through improving cell antioxidant ablility.

关 键 词：阿替洛尔 过氧化氢 心肌细胞 

分 类 号：R542.2[医药卫生—心血管疾病]