高效液相色谱法测定升白合剂中淫羊藿苷的含量  

Determination of icariine in Shengbai mixture by HPLC

作　　者：张虹[1] 郑捷[1] 叶显撑[1] 吴涓[1] 方昱[1] 

机构地区：[1]同济大学附属同济医院,上海200065

出　　处：《中国医院药学杂志》2007年第7期872-874,共3页Chinese Journal of Hospital Pharmacy

基　　金：上海市科委资助项目(编号:02DZ19134)

摘　　要：目的：建立高效液相色谱（HPLC）法测定升白合剂中淫羊藿苷的含量。方法：色谱柱Diamonsil^TM C18柱（250mm×4．6mm，5μm），流动相为甲醇-水-醋酸（60：40：0.4），检测波长为270nm，柱温为25℃，流速为1.0mL·min。结果：精密度及稳定性均良好；在18～90mg·L^-1的范围，峰面积与浓度呈良好的线性关系（r=0．9999），平均回收率为98.25％，日内精密度RSD为0.60％～6.99％，日间精密度RSD为1.61％～5．83％。结论：本方法简便、快速、准确、重复性好，可用于升白合剂中淫羊藿苷的含量测定。OBJECTIVE To establish an HPLC method for the determination of icariine in Shengbai mixture. METHODS The separation of icariine was achieved on a DiamonsilTM C18 column（250 mm × 4. 6 mm, 5 μm）. The mobile phase was composed of methanol-water-acetic acid （60：40：0. 4） at a flow rate of 1.0 mL·min^-1. The detection wavelength was 270 nm,and column temperature 25 ℃. RESULTS The calibration curve was linear in the range of 18 to 90 mg·L-1 ,and the correlation coefficient was 0. 999 9. The average recovery was 98. 25%. The precision, expressed as the relative standard deviation （RSD） in a day and between days, was 0. 60%-6. 99% and 1.61%-5.83% respectively. CONCLUSION The HPLC method for the determination of icariine in shengbai mixture is simple, rapid, accurate and reliable, which is suited for the detemination of icariiue in shengbai mixture.

关 键 词：淫羊藿苷 高效液相色谱法 升白合剂 

分 类 号：R927.2[医药卫生—药学]