东亚七筋姑多倍体ITS区的序列测定与分析  

Clone and Sequence Analysis of rDNA ITS of Clintonia udensis

作　　者：郭志刚[1] 刘占林[1] 王祎玲[1] 李思锋[2] 赵桂仿[1] 

机构地区：[1]西北大学生命科学学院西部资源生物与现代生物技术教育部重点实验室,西安710069 [2]西安植物园,西安710061

出　　处：《西北植物学报》2008年第5期914-921,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：教育部博士点基金项目(20040697010);国家自然科学基金项目(30670129);陕西省自然科学基金项目(2005C117)

摘　　要：通过对13个东亚七筋姑二倍体居群和5个四倍体居群的ITS区（包括ITS1、5．8S rDNA和ITS2）进行克隆测序。ITS序列长度显示出比较高的多态性，ITS1长度为171～270bp，ITS2长度为205～238bp，5．8S序列的长度在162～164bp之间，序列中G＋C含量为45．83％～54．13％，当空位（gap）作缺失处理时，东亚七筋姑ITS区全序列排序后的长度为682bp，其中简约性位点289个；最大简约法分析获得一致性指数（CI）为0．630，保留系数（RI）为0．684，四倍体居群没有单独聚为一支，而是和相邻地理单元的二倍体居群聚为一支，本研究结果支持东亚七筋姑异地多起源的推断。Clintonia udensis belongs to Liliaceae. ITS sequences of 13 C. udensis diploid populations and 5 tetraploid populations were reported by cloning and sequencing. Sequences analysis showed that ITS1 is 171-270 bp,ITS2 is 205-238 bp and 5.8S is 162-164 bp. Among the 18 ITS sequences 289 site were informative. The contents of G＋C of the sequences were between 45.83%-54.13%. Phylogenetic analysis of ITS sequences obtained the strict consensus trees （Length=1 056,CI=0. 630 and RI=0. 684） when the gaps were treated as deletion. The result indicated that the diploid populations and tetraploid populations from same region was always clustered. The origin of C. udensis polyploidy was polytypic.

关 键 词：东亚七筋姑 ITS 多倍体 

分 类 号：Q789[生物学—分子生物学]