免疫金银染色法检测SLE患者血清ANA和抗-dsDNA抗体  

Detection of ANA and Anti-dsDNA antibodies in the sera of patients with SLE by IGSS

作　　者：张铁汉[1] 赵永新[1] 张帮杰[1] 刘晓军[1] 王伟娟[1] 

机构地区：[1]河南省新乡医学院第三附属医院,453003

出　　处：《中国实用医药》2008年第15期24-26,共3页China Practical Medicine

摘　　要：目的探讨免疫金银染色法检测抗核抗体(ANA)、抗dsDNA抗体对诊断系统性红斑狼疮(SLE)的价值。方法采用免疫金银染色法(IGSS)检测SLE患者血清中ANA和抗-dsDNA。结果活动期ANA、抗-dsDNA阳性率与非活动期比较差异有统计学意义(P<0·01)。活动期抗dsDNA平均滴度明显高于非活动期,抗dsDNA阳性组肾损害明显高于抗dsDNA阴性者。(P<0·01)。结论免疫金银染色法检测ANA、抗dsDNA有高度特异性、敏感性和稳定性,可在基层医院开展。Objective To investigate the value of detect the anti-nuclear antibody（ANA）and anti-double stranded DNA（A-dsDNA）in the sera of patients with SLE.Methods The ANA and A-dsDNA antibodies in the sera of 86 patients with SLE were detected by using immunogold-silver staining（IGSS）.Results The difference in the positive rates of ANA and A-dsDNA was significant between the active and inactive cases（P〈0.01）,and the titers of ANA in active cases were higher than that in the inactive cases（P〈0.01）.The incidence of renal damage in patients with positive A-dsDNA was higher than that in patients with negative A-dsDNA.Conclusion The IGSS is highly sensitive and reliable for detecting ANA and A-dsDNA in patients with SLE.And the higher titer of A-dsDNA suggest the possibility of renal damage.The IGSS is more simple and rapid for application to small medical laboratories.

关 键 词：系统性红斑狼疮 抗核抗体 抗DSDNA抗体 免疫金银染色 

分 类 号：R593.241[医药卫生—内科学] R446.62[医药卫生—临床医学]