七氟醚后处理对局灶性脑缺血-再灌注损伤的保护作用  

The protective effect of sevoflurane postconditioning on focal cerebral ischemia reperfusioon injury

作　　者：夏明[1] 董海龙[1] 谢克亮[1] 熊利泽[1] 

机构地区：[1]第四军医大学西京医院麻醉科,西安市710032

出　　处：《临床麻醉学杂志》2008年第5期434-436,共3页Journal of Clinical Anesthesiology

基　　金：国家自然科学基金资助(30471664)

摘　　要：目的评价在缺血后期及再灌注早期吸入不同浓度的七氟醚行后处理对大鼠局灶性脑缺血-再灌注损伤的保护作用及其剂量依赖性。方法雄性SD大鼠50只,随机分为空白对照组、吸氧组和0.5、1.0、1.5MAC七氟醚后处理组,每组10只。采用大脑中动脉线栓法阻闭(middle cerebral artery occlusion,MCAO)120min后再灌注72h制备局灶性脑缺血模型。各七氟醚后处理组于再灌注即刻的前20min和后10min给予不同浓度七氟醚吸入。再灌注后的24、48和72h行神经功能评分(NDS),并于最后一次评分后测定脑梗死容积比。结果0.5、1.0和1.5MAC组的脑梗死容积比分别为0.39±0.03,0.31±0.03和0.24±0.03(P<0.05),明显小于对照组0.53±0.05(P<0.05)。吸氧组为0.51±0.05,与对照组比差异无统计学意义。各个时间点七氟醚后处理组NDS明显优于对照组和吸氧组。结论在局灶性脑缺血-再灌注的缺血后期和再灌注早期吸入0.5、1.0和1.5MAC七氟醚行后处理均具有脑保护作用,并呈现剂量依赖性。Objective To investigate the protective effect of sevoflurane postconditioning with different concentrations given at the onset of reperfusion on focal cerebral ischemic-reperfusion injury. Methods Fifty male SD rats were randomly assigned to five groups （with 10 rats each）： control group,oxygen inhalation group and 0.5 MAC, 1, 0 MAC, 1, 5 MAC sevoflurane postconditioning group. All animals were subjected to the right middle cerebral artery occlusion（MCAO） for 120 rain, followed by reperfusion for 72 h. The animals in 0.5,1.0 and 1.5 MAC group were given 0.5,1.0 and 1.5 MAC sevoflurane inhalation from 20 min before to 10 min after reperfusion. The neurological deficit scores （NDS） were recorded at 24,48 ,and 72 h after reperfusion. Infarct volume percentage was determined after the last NDS assessment. Results The infarct volume percentage ratio of 0.5,1.0 and 1.5 MAC group were 0.39±0.03,0.31±0.03 and 0. 24 ±0. 03 , respectively （P〈0.05, among the groups）, which were significantly smaller than 0.53 ± 0.05 in control group （P〈 0.05）. The infarct volume percentage of O2 group was 0.51±0.05 ,which was not significantly different with that of control group. NDS of 24,48 h and 72 h after reperfusion in all sevoflurane groups was significantly higher than that in control and oxygen groups. Conclusion 0.5, 1.0 and 1.5 MAC sevoflurane postconditioning has a significant neuroprotective effect in a dose-dependent manner.

关 键 词：七氟醚 局灶性 脑缺血 再灌注损伤 

分 类 号：R614[医药卫生—麻醉学]